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Cited by 6Photoreceptor and optogenetics researchProtein Structure and DynamicsReceptor Mechanisms and Signaling

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Engineering of soluble bacteriorhodopsin

Open access
Nikolaev, Andrey, Orlov, Yaroslav, Tsybrov, Fedor, Kuznetsova, Elizaveta, Shishkin, Pavel, Kuzmin, Alexander, Mikhailov, Anatolii, Nikolaeva, Yulia S., Anuchina, Arina, Chizhov, Igor, Semenov, Oleg, Kapranov, Ivan, Borshchevskiy, Valentin, Remeeva, Alina, Gushchin, Ivan·2025·Chemical Science
DOI 10.1039/d5sc02453fPMC PMC12094106

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Abstract

Analogues of membrane protein bacteriorhodopsin are reported that are soluble and stable, bind retinal and exhibit photocycles under illumination, highlighting the power of modern protein engineering approaches.

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Metadata

Authors
Nikolaev, A., Orlov, Y., Tsybrov, F., Kuznetsova, E., Shishkin, P., Kuzmin, A., Mikhailov, A., Nikolaeva, Y. S., Anuchina, A., Chizhov, I., Semenov, O., Kapranov, I., Borshchevskiy, V., Remeeva, A., & Gushchin, I.
Affiliations
Moscow Institute of Physics and Technology (RU)
Corresponding author
Gushchin, Ivan
ORCID
Venue
Chemical Science
Publisher
Royal Society of Chemistry (RSC)
Published
2025
Volume
16
Issue
24
Pages
11067-11076
ISSN
  • 2041-6520 (ISSN-L)
  • 2041-6539
DOI
10.1039/d5sc02453f
PMC
PMC12094106
OpenAlex
W4410338897
Cited by
6
References
3
Citations by year
2026: 5 · 2025: 1
FWCI
2.35
Type
journal article
Language
en
Open access
Licence
Locations
  • Chemical Science (publishedVersion, cc-by-nc)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
  • Russian Science Foundation (21-64-00018)
  • Ministry of Science and Higher Education of the Russian Federation (075-15-2021-1354, FSMG-2025-0003)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
Keywords
bacteriorhodopsin, retinal binding pocket, chromophore binding, photocycle, crystal structure, optogenetics, light-driven proton pump

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