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The estimation of creatine and of diacetyl
Open accessAbstract
Voges & Proskauer [1898] observed that when strong alkali was added to broth cultures of certain species of bacteria there developed, after an interval of time, a pink colour.Subsequently the work of Harden [1906] and of Harden & Norris [1911] established that the chemical reaction responsible was one between diacetyl and creatine (or certain similar substances).Attempts to make the reaction quantitative [Walpole, 1911; Eggleton & Eggleton, 1928] were only partially successful.The reaction was further studied by Duliere [1929], Lang [1932] and Muller [1935], but it was the discovery by Barritt [1936], that o-naphthol greatly intensifies the colour, which made it possible for the first timne to use this reaction as a means of estimating either creatine or diacetyl.The present paper presents some additional facts about the reaction and methods of estimation based on it. EXPERIMENTAL Preliminary experiment
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Cited by
Mechanochemical coupling in actomyosin energy transduction studied by in vitro movement assay
Harada, Y., Sakurada, K., Aoki, T., Thomas, D. D., & Yanagida, T. (1990) · Journal of Molecular Biology · doi:10.1016/s0022-2836(05)80060-9 · Cited by 522
Creatine Kinase of Rat Heart Mitochondria
Jacobus, W. E., & Lehninger, A. L. (1973) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)43737-x · Cited by 469
Metadata
- Authors
- Eggleton, P., Elsden, S. R., & Gough, N.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1943-11-01
- Volume
- 37
- Issue
- 5
- Pages
- 526-529
- ISSN
- 0306-3283 (ISSN-L)
- OpenAlex
- W2412626467
- Cited by
- 255
- References
- 0
- FWCI
- 0.85
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Eggleton, P., Elsden, S. R., & Gough, N. (1943). The estimation of creatine and of diacetyl. Biochemical Journal. https://doi.org/10.1042/bj0370526
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