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Rat intestinal microvillus membranes. Purification and biochemical characterization
Open accessAbstract
1. A technique is described for the removal of subcellular contaminants from intact rat intestinal brush borders, and for the subsequent separation of a microvillus membrane fraction from a fibrillar residue. 2. Increments in invertase activity, microscopic homogeneity and low nucleic acid content indicate that the microvillus plasma membrane has been extensively purified. Multiple membrane preparations have been shown to be highly reproducible with respect to their invertase specific activity, cholesterol content and phospholipid content. Alkaline phosphatase, leucine aminopeptidase, Mg2+- and Ca2+-dependent adenosine triphosphatase and seven separate disaccharidases were shown to be predominantly confined to the membrane fraction. 3. The fibrillar fraction has been shown to contain approximately 30% of the total protein of purified brush borders, plus most of the residual nucleic acid contaminant. No evidence was found for the localization of any specific enzyme in this fraction.
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Citations
Cited by
Avruch, J., & Hoelzl Wallach, D. F. (1971) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(71)90331-2 · Cited by 828
Synaptic plasma membranes from rat brain synaptosomes: Isolation and partial characterization
Cotman, C. W., & Matthews, D. A. (1971) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(71)90117-9 · Cited by 629
Mooseker, M. S., & Tilney, L. G. (1975) · The Journal of cell biology · doi:10.1083/jcb.67.3.725 · Cited by 622
The aminopeptidase from hog intestinal brush border
Maroux, S., Louvard, D., & Barath, J. (1973) · Biochimica et Biophysica Acta (BBA) - Enzymology · doi:10.1016/0005-2744(73)90083-1 · Cited by 537
Ogawa, N., Satsu, H., Watanabe, H., Fukaya, M., Tsukamoto, Y., Miyamoto, Y., & Shimizu, M. (2000) · The Journal of Nutrition · doi:10.1093/jn/130.3.507 · Cited by 110
Metadata
- Authors
- Forstner, G. G., Sabesin, S. M., & Isselbacher, K. J.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1968-01-01
- Volume
- 106
- Issue
- 2
- Pages
- 381-390
- ISSN
- 0306-3283 (ISSN-L)
- OpenAlex
- W1802229984
- Cited by
- 452
- References
- 0
- FWCI
- 25.31
- Type
- journal article
- Language
- en
- Open access
- green
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- Forstner, G. G., Sabesin, S. M., & Isselbacher, K. J. (1968). Rat intestinal microvillus membranes. Purification and biochemical characterization. Biochemical Journal. https://doi.org/10.1042/bj1060381
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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