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An enzymic method for the trace iodination of immunoglobulins and other proteins
Open accessAbstract
1. A method is described for the trace iodination of immunoglobulins and other serum proteins by a system consisting of lactoperoxidase, hydrogen peroxide and iodide. 2. γG immunoglobulin that had been labelled to a specific radioactivity of 5μc/μg. by use of carrier-free [125I]iodide gave no evidence of denaturation when analysed by electrophoresis and density-gradient ultracentrifugation. 3. Tryptic hydrolysis and peptide ‘mapping’ of a completely characterized peptide radioiodinated by this method showed that the [125I]iodide was bound to tyrosyl residues. 4. Proteins differ in their susceptibility to iodination by this method. Human γG immunoglobulin, for example, is iodinated more than ten times as readily as is human α2-macroglobulin under the same conditions. 5. Lactoperoxidase catalyses the iodination of proteins much more readily than does horseradish peroxidase.
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Cited by
Bolton, A. E., & Hunter, W. M. (1973) · Biochemical Journal · doi:10.1042/bj1330529 · Cited by 3,254
Enzymatic iodination of polypeptides with 125I to high specific activity
Thorell, J. I., & Johansson, B. G. (1971) · Biochimica et Biophysica Acta (BBA) - Protein Structure · doi:10.1016/0005-2795(71)90123-1 · Cited by 1,156
Altered chloride ion channel kinetics associated with the ΔF508 cystic fibrosis mutation
Dalemans, W., Barbry, P., Champigny, G., Jallat, S., Jallat, S., Dott, K., Dreyer, D., Crystal, R. G., Pavirani, A., Lecocq, J.-P., et al. (1991) · Nature · doi:10.1038/354526a0 · Cited by 707
Yarden, Y., & Schlessinger, J. (1987) · Biochemistry · doi:10.1021/bi00379a035 · Cited by 627
In vivo neurotrophic effects of GDNF on neonatal and adult facial motor neurons
Yan, Q., Matheson, C., & Lopez, O. T. (1995) · Nature · doi:10.1038/373341a0 · Cited by 592
Metadata
- Authors
- Marchalonis, J. J.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1969-06-01
- Volume
- 113
- Issue
- 2
- Pages
- 299-305
- ISSN
- 0306-3283 (ISSN-L)
- OpenAlex
- W1553230769
- Cited by
- 1,397
- References
- 0
- FWCI
- 22.69
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Marchalonis, J. J. (1969). An enzymic method for the trace iodination of immunoglobulins and other proteins. Biochemical Journal. https://doi.org/10.1042/bj1130299
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