The DOI matches its Crossref record; 16 of the 17 references it cites were found in a registry or an index.
Role of calcium ions in the regulation of intramitochondrial metabolism. Properties of the Ca2+-sensitive dehydrogenases within intact uncoupled mitochondria from the white and brown adipose tissue of the rat
Open accessAbstract
1. Increasing concentrations of both Ca2+ and Sr2+ (generated by using EGTA buffers) resulted in 4-fold increases in the initial activity of pyruvate dehydrogenase within intact uncoupled mitochondria from rat epididymal adipose tissue incubated in the presence of the ionophore A23187, ATP, Mg2+ and oligomycin. The k0.5 values (concentrations required for half-maximal effects) for Ca2+ and Sr2+ were 0.54 and 7.1 microM respectively. In extracts of the mitochondria, pyruvate dehydrogenase phosphate phosphatase activity was stimulated about 4-fold by Ca2+ and Sr2+, with k0.5 values of 1.08 and 6.4 microM respectively. 2. NAD+-isocitrate dehydrogenase and oxoglutarate dehydrogenase appeared to be rate-limiting in the oxidation of threo-Ds-isocitrate and oxoglutarate by uncoupled mitochondria from brown adipose tissue of cold-adapted rats. Ca2+ (and Sr2+) diminished the Km for the oxidation of both threo-Ds-isocitrate and oxoglutarate. The kinetic constants for these oxidations were very similar to those obtained for the activities of NAD+-isocitrate dehydrogenase and oxoglutarate dehydrogenase in extracts of the mitochondria. In particular, the k0.5 values for Ca2+ were all in the range 0.2–1.6 microM and Sr2+ was found to mimic Ca2+, but with k0.5 values about 10 times greater. 3. Overall, the results of this study demonstrate that the activities of pyruvate dehydrogenase, NAD+-isocitrate dehydrogenase and oxoglutarate dehydrogenase may all be increased by Ca2+ and Sr2+ within intact mitochondria. In all cases the k0.5 values are close to 1 and 10 microM respectively, as found for the separated enzymes. Experiments on brown-adipose-tissue mitochondria incubated in the presence of albumin suggest that it may be possible to use the sensitivity of the dehydrogenases to Ca2+ as a means of assessing the distribution of Ca2+ across the mitochondrial inner membrane.
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“Support for a role of ROS in HIF-1α stabilization derives from studies showing that the absence of cytochrome c or blocked Rieske Fe-S proteins under hypoxic conditions blocks HIF-1α stabilization (61, 221).”
From Mitochondrial reactive oxygen species production in excitable cells: Modulators of mitochondrial and cell function (2009) · cites this paper as [221]
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Removal of Fatty Acids from Serum Albumin by Charcoal Treatment
Chen, R. F. (1967) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)81445-x · Cited by 2,093
The Respiratory Chain and Oxidative Phosphorylation
Chance, B., & Williams, G. R. (1956) · Advances in enzymology and related areas of molecular biology/Advances in enzymology and related subjects · doi:10.1002/9780470122624.ch2 · Cited by 2,084
Stimulation by calcium ions of pyruvate dehydrogenase phosphate phosphatase
Denton, R. M., Randle, P. J., & Martin, B. R. (1972) · Biochemical Journal · doi:10.1042/bj1280161 · Cited by 542
Self-citationMcCORMACK, J. G., & Denton, R. M. (1979) · Biochemical Journal · doi:10.1042/bj1800533 · Cited by 453
Self-citationDenton, R. M., Richards, D. A. R., & Chin, J. (1978) · Biochemical Journal · doi:10.1042/bj1760899 · Cited by 375
Self-citationRegulation of mammalian pyruvate dehydrogenase
Denton, R. M., Randle, P. J., Bridges, B. J., Cooper, R. H., Kerbey, A. L., Pask, H. T., Severson, D. L., Stansbie, D., & Whitehouse, S. (1975) · Molecular and Cellular Biochemistry · doi:10.1007/bf01731731 · Cited by 304
Self-citationDenton, R. M., McCormack, J. G., & EDGELL, N. J. (1980) · Biochemical Journal · doi:10.1042/bj1900107 · Cited by 304
Self-citationRegulation of heart muscle pyruvate dehydrogenase kinase
Cooper, R. H., Randle, P. J., & Denton, R. M. (1974) · Biochemical Journal · doi:10.1042/bj1430625 · Cited by 231
Self-citation[8] Mitochondria from brown adipose tissue: Isolation and properties
Cannon, B., & Lindberg, O. (1979) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(79)55010-1 · Cited by 224
Regulation of pyruvate metabolism in mammalian tissues.
Denton, R. M., & Halestrap, A. P. (1979) · PubMed · Cited by 170
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Research works printed but not linked: 1
- Plaut, G. W. E. & Smith, C. M. (1977) in PIvridine Nucleotide-Depenideni DehYdrogenases (Sund. H.. ed.), pp. 444-457, Walter de Gruyter, Berlin
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Glancy, B. C., & Balaban, R. S. (2012) · Biochemistry · doi:10.1021/bi2018909 · Cited by 649
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Carafoli, E., Santella, L., Branca, D., & Brini, M. (2001) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.1080/20014091074183 · Cited by 524
Mitochondrial calcium transport
Nicholls, D. G., & Åkerman, K. E. O. (1982) · Biochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics · doi:10.1016/0304-4173(82)90013-1 · Cited by 486
Stowe, D. F., & Camara, A. K. S. (2009) · Antioxidants & Redox Signaling · doi:10.1089/ars.2008.2331 · Cited by 471
Metadata
- Authors
- McCormack, J. G., & Denton, R. M.
- Affiliations
- University of Bristol (GB)
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1980-07-15
- Volume
- 190
- Issue
- 1
- Pages
- 95-105
- ISSN
- 0264-6021 (ISSN-L)
- PMC
- PMC1162067
- OpenAlex
- W130501137
- Cited by
- 165
- References
- 17
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 5 · 2023: 3 · 2022: 4 · 2021: 6 · 2020: 2 · 2019: 2 · 2018: 5 · 2017: 3 · 2016: 1 · 2015: 2 · 2013: 4 · 2012: 3
- FWCI
- 5.68
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Biochemical Acid Research Studies
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Keywords
- calcium ions, pyruvate dehydrogenase, NAD-isocitrate dehydrogenase, 2-oxoglutarate dehydrogenase, strontium, white adipose tissue, brown adipose tissue, cold adaptation, substrate Km, A23187
- MeSH terms
- Calcimycin, Adenosine Triphosphate, Adipose Tissue, Animals, Adipose Tissue, Brown, Calcium, Kinetics, Male, Mitochondria, Oxidoreductases, Serum Albumin, Bovine, Strontium, Rats, In Vitro Techniques
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