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Alkaline O→N-transacylation. A new method for the quantitative deacylation of phospholipids
Open accessAbstract
1. Quantitative O-deacylation of phospholipids has been achieved by incubation with a reagent containing monomethylamine, methanol and water. The reaction is primarily an O leads to N-transacylation with N-methyl fatty acid amides being formed. 2. The reagent can be removed easily by volatilization and under defined conditions no secondary decomposition of the phosphorus-containing deacylation products occurs. 3. The water-soluble phosphorus compounds derived by deacylation of mammalian tissue O-diacylated phospholipids have been completely separated by a single-dimensional paper ionophoresis with a volatile pH9 buffer. 4. The O-deacylated alkyl and alkenyl phospholipids have been examined by t.l.c. before and after catalytic hydrolysis with Hg2+. 5. A complete analysis of rat brain phospholipids by the above methods agrees closely with that obtained by other procedures.
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Citations
Cited by
Oh, C.-S., Toke, D. A., Mandala, S., & Martin, C. E. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.28.17376 · Cited by 486
Sphingolipid Synthesis as a Target for Antifungal Drugs
Nagiec, M. M., Nagiec, E. E., Baltisberger, J. A., Wells, G. B., Lester, R. L., & Dickson, R. C. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.15.9809 · Cited by 391
Volinia, S., Dhand, R., Vanhaesebroeck, B., MacDougall, L. K., Stein, R., Zvelebil, M. J., Domin, J., Panaretou, C., & Waterfield, M. D. (1995) · The EMBO Journal · doi:10.1002/j.1460-2075.1995.tb07340.x · Cited by 307
Lipid abnormalities in atopic skin are driven by type 2 cytokines
Berdyshev, E., Goleva, E., Bronova, I., Dyjack, N., Rios, C., Jung, J., Taylor, P., Jeong, M., Hall, C. F., Richers, B. N., et al. (2018) · JCI Insight · doi:10.1172/jci.insight.98006 · Cited by 283
Somm, E., Montandon, S. A., Loizides-Mangold, U., Gaïa, N., Lazarevic, V., De Vito, C., Perroud, E., Bochaton-Piallat, M.-L., Dibner, C., Schrenzel, J., et al. (2021) · Translational Research · doi:10.1016/j.trsl.2020.07.008 · Cited by 118
Metadata
- Authors
- Clarke, N. G., & Dawson, R. M. C.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1981-04-01
- Volume
- 195
- Issue
- 1
- Pages
- 301-306
- ISSN
- 0264-6021 (ISSN-L)
- OpenAlex
- W1768606565
- Cited by
- 318
- References
- 0
- FWCI
- 3.40
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Alkaline O→N-transacylation. A new method for the quantitative deacylation of phospholipids. https://app.cochat.ai/papers/10.1042/bj1950301
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- Clarke, N. G., & Dawson, R. M. C. (1981). Alkaline O→N-transacylation. A new method for the quantitative deacylation of phospholipids. Biochemical Journal. https://doi.org/10.1042/bj1950301
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
