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Cited by 339

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Hyaluronate is synthesized at plasma membranes

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Prehm, P·1984·Biochemical Journal
DOI 10.1042/bj2200597
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Abstract

The hybrid cell B6 line, which synthesizes large amounts of hyaluronate as the predominant glycosaminoglycan, was grown in the presence of [3H]glucosamine. The [3H]hyaluronate has a high molecular weight and was excluded by Sephacryl S-1000. After disruption of the cells the [3H]hyaluronate could further be elongated by incubation with UDP-GlcNAc and UDP-[14C]GlcA, yielding a hybrid molecule of hyaluronate labelled with [3H]GlcNAc and [14C]GlcA. Treatment of the cells with hyaluronidase before disruption eliminated the large [3H]hyaluronate and elongation of nascent chains in vitro commenced from low-molecular-weight chains. Thus nascent hyaluronate chains were degraded extracellularly by hyaluronidase and were therefore synthesized at the inner side of plasma membranes and extruded to the cell surface.

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Metadata

Authors
Prehm, P.
Venue
Biochemical Journal
Publisher
Portland Press Ltd.
Published
1984-06-01
Volume
220
Issue
2
Pages
597-600
ISSN
  • 0264-6021 (ISSN-L)
  • 1470-8728
DOI
10.1042/bj2200597
OpenAlex
W348026187
Cited by
339
References
0
FWCI
3.66
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Hyaluronate is synthesized at plasma membranes. https://app.cochat.ai/papers/10.1042/bj2200597
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Prehm, P. (1984). Hyaluronate is synthesized at plasma membranes. Biochemical Journal. https://doi.org/10.1042/bj2200597

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.