Cited by 53RNA and protein synthesis mechanismsCellular transport and secretionChemical Synthesis and Analysis

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Design and synthesis of a consensus signal sequence that inhibits protein translocation into rough microsomal vesicles.

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Austen, B M, Hermon-Taylor, J, Kaderbhai, M A, Ridd, D H·1984·Biochemical Journal

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Abstract

Most signal sequences are found to vary considerably in length and primary sequence, but possess some common structural features. Analysis of known signal sequences has led to the design of a 19-residue sequence that, although not a naturally occurring signal, possesses the structural features that commonly occur in pre-proteins. This peptide has been synthesized by solid-phase methods, and has been shown to inhibit, in a concentration-dependent manner, the processing in vitro of nascent pre-prolactin, pre-forms of pancreatic digestive enzymes, and pre-placental lactogen. The peptide acts at the cytoplasmic surface of microsomal vesicles added to the protein translation system, preventing translocation of the nascent chains to the lumenal space of vesicles where signal peptidase normally cleaves to remove the signal from nascent pre-proteins.

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Authors: Austen, B. M., Hermon-Taylor, J., Kaderbhai, M. A., & Ridd, D. H.
Affiliations: St George's, University of London (GB)
Venue: Biochemical Journal
Publisher: Portland Press Ltd.
Published: 1984-11-15
Volume: 224
Issue: 1
Pages: 317-325
ISSN: 0264-6021 (ISSN-L)
1470-8728
DOI: 10.1042/bj2240317
PMC: PMC1144429
OpenAlex: W2129132769
Cited by: 53
References: 29
Citations by year: 2021: 1 · 2015: 1
FWCI: 2.43
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: Biochemical Journal (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
Physical Sciences › Chemistry › Organic Chemistry › Chemical Synthesis and Analysis
Keywords: signal peptide, protein translocation, signal peptidase, microsomal vesicles, prolactin, pancreatic enzymes, placental lactogen, signal sequence cleavage
MeSH terms: Amino Acid Sequence, Animals, Cattle, Dogs, Electrophoresis, Polyacrylamide Gel, Microsomes, Models, Molecular, Peptides, Placental Lactogen, Prolactin, Protein Precursors, Proteins, Protein Sorting Signals, In Vitro Techniques

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Design and synthesis of a consensus signal sequence that inhibits protein translocation into rough microsomal vesicles.

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