The DOI matches its Crossref record.
Biotin binding to avidin. Oligosaccharide side chain not required for ligand association
Open accessAbstract
A commercially available, purified preparation of avidin was found to comprise two polypeptide bands (Mr 18,000 and Mr 15,500 respectively). Both bands bound biotin as assessed by biotin overlays of protein blots. The Mr 15,500 polypeptide was found to differ from the Mr 18,000 polypeptide only in its sugar content. When the commercial preparation was applied to a concanavalin A affinity column, the glycosylated forms were retarded as expected, and homotypic nonglycosylated avidin tetramers which failed to bind selectively to the column were collected in the effluent. The biotin-binding properties of the nonglycosylated avidin were equivalent to those obtained for the native (glycosylated) avidin molecule, indicating that the oligosaccharide moiety is not essential for the binding activity.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Spin Trapping: ESR parameters of spin adducts 1474 1528V
Buettner, G. R. (1987) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(87)80033-3 · Cited by 1,827
The avidin-biotin complex in bioanalytical applications
Wilchek, M., & Bayer, E. A. (1988) · Analytical Biochemistry · doi:10.1016/0003-2697(88)90120-0 · Cited by 894
Self-citationPinaud, F., King, D., Moore, H.-P., & Weiss, S. (2004) · Journal of the American Chemical Society · doi:10.1021/ja031691c · Cited by 573
Target Identification for Small Bioactive Molecules: Finding the Needle in the Haystack
Ziegler, S., Pries, V., Hedberg, C., & Waldmann, H. (2013) · Angewandte Chemie International Edition · doi:10.1002/anie.201208749 · Cited by 466
Huang, N.-P., Vörös, J., De Paul, S. M., Textor, M., & Spencer, N. D. (2002) · Langmuir · doi:10.1021/la010913m · Cited by 268
Metadata
- Authors
- Hiller, Y., Gershoni, J. M., Bayer, E. A., & Wilchek, M.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1987-11-15
- Volume
- 248
- Issue
- 1
- Pages
- 167-171
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- OpenAlex
- W1788023629
- Cited by
- 165
- References
- 0
- FWCI
- 2.97
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Biotin binding to avidin. Oligosaccharide side chain not required for ligand association. https://app.cochat.ai/papers/10.1042/bj2480167
- The paper
- Hiller, Y., Gershoni, J. M., Bayer, E. A., & Wilchek, M. (1987). Biotin binding to avidin. Oligosaccharide side chain not required for ligand association. Biochemical Journal. https://doi.org/10.1042/bj2480167
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
