Restricted diffusion of integral membrane proteins and polyphosphoinositides leads to their depletion in microvesicles released from human erythrocytes
Open accessAbstract
The protein and phospholipid composition of microvesicles released from normal human erythrocytes after ATP depletion, on aging or by treatment with merocyanine 540, dimyristoyl phosphatidylcholine or Ca2+/ionophore A23187 has been compared with the composition of the original cell membrane. It has been shown that these microvesicles are depleted of band 3, glycophorin and phosphatidylinositol 4,5-bisphosphate relative to phospholipid by 40% or more. These data are interpreted to mean that less than half of these membrane components are free to diffuse laterally in the lipid bilayer. Acetylcholinesterase was found to be enriched 2-3-fold in microvesicles, possibly because the removal of non-diffusing proteins from the vesiculating region of the lipid bilayer allows more space for freely diffusing proteins like acetylcholinesterase to enter the microvesicle membrane.
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In red blood cells, 50% of PI(4,5)P2 is bound to cytoskeletal proteins.
“Similar results have been seen in red blood cells where 50% of the PI(4,5)P2 is bound to cytoskeletal proteins [125].”
From Phosphatidylinositol(4,5)bisphosphate: diverse functions at the plasma membrane (2020) · cites this paper as [125]
In 'Partitioning of polyphosphoinositides into microvesicles' (page unavailable), the paper reports that microvesicles contain about half as much polyphosphoinositide as the original cells, suggesting that only about half is free to diffuse. It proposes binding to cytoskeletal proteins as a possible explanation; it does not establish that 50% of PI(4,5)P2 is bound to them. (LLM, conf=0.88)
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Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751 · see citing works
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Research works printed but not linked: 4
- Rumbsby, M. G., Trotter, J., Allan, D. & Michell, R. H. (1977) Biochem. Soc. Trans. 5, 126-128
- Nelson, G. J. (1972) in Blood Lipids and Lipoproteins: Quantitation, Composition and Metabolism (Nelson, G. J., ed.), pp. 317-318, Interscience, New York
- Sheetz, M. P. & Singer, S. J. (1974) Proc. Natl. Acad. Sci. U.S.A. 71, 4457-4461
- Nigg, E. A. & Cherry, R. J. (1980) Proc. Natl. Acad. Sci. U.S.A. 77, 4702-4706
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Phosphatidylinositol(4,5)bisphosphate: diverse functions at the plasma membrane
Katan, M., & Cockcroft, S. (2020) · Essays in Biochemistry · doi:10.1042/ebc20200041 · Cited by 176 · see citing works
- Authors
- Hagelberg, C., & Allan, D.
- Affiliations
- University College London (GB)
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1990-11-01
- Volume
- 271
- Issue
- 3
- Pages
- 831-834
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- PMC
- PMC1149640
- OpenAlex
- W2271388588
- Cited by
- 72
- References
- 1
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 1 · 2020: 3 · 2018: 1 · 2017: 4 · 2016: 1 · 2015: 2 · 2012: 4
- FWCI
- 1.86
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Health Sciences › Medicine › Physiology › Erythrocyte Function and Pathophysiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- membrane protein diffusion, band 3, glycophorin, phosphatidylinositol 4,5-bisphosphate, polyphosphoinositides, acetylcholinesterase, membrane lipid composition, ATP depletion, merocyanine 540, A23187, membrane aging, vesicle formation
- MeSH terms
- Calcimycin, Anion Exchange Protein 1, Erythrocyte, Calcium, Diffusion, Dimyristoylphosphatidylcholine, Erythrocytes, Humans, Lipid Bilayers, Lipids, Phosphatidylinositols, Pyrimidinones, Radiation-Sensitizing Agents, Phosphatidylinositol Phosphates
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