The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Human cathepsin K cleaves native type I and II collagens at the N-terminal end of the triple helix
Open accessAbstract
Cathepsin K (EC 3.4.22.38) is a recently described enzyme that has been shown to cleave type I collagen in its triple helix. The aim of this study was to determine if it also cleaves type II collagen in the triple helix and to identify the helical cleavage site(s) in types I and II collagens. Soluble human and bovine type II collagen, and rat type I collagen, were incubated with cathepsin K before the reaction was stopped with trans-epoxysuccinyl-l-leucylamido-(4-guanidino)butane (E-64). Analysis by SDS/PAGE of the collagen digests showed that optimal activity of cathepsin K against native type II collagen was between pH 5.0 and 5.5 and against denatured collagen between pH 4.0 and 7.0. The enzyme cleaved telopeptides as well as the α1(II) chains, generating multiple fragments in the range 90–120 kDa. The collagenolytic activity was not due to a contaminating metalloenzyme or serine proteinase as it was not inhibited by 1,10-phenanthroline, EDTA or 3,4-dichloroisocoumarin. Western blotting with anti-peptide antibodies to different regions of the α1(II) chain suggested that cathepsin K cleaved native α1(II) chains in the N-terminal region of the helical domain rather than at the well-defined collagenase cleavage site. This was confirmed by N-terminal sequencing of one of the fragments, revealing cleavage at a Gly-Lys bond, 58 residues from the N-terminus of the helical domain. By using a similar approach, cathepsin K was found to cleave native type I collagen close to the N-terminus of its triple helix. These results indicate that cathepsin K could have a role in the turnover of type II collagen, as well as type I collagen.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,221
Pycnodysostosis, a Lysosomal Disease Caused by Cathepsin K Deficiency
Gelb, B. D., Shi, G.-P., Chapman, H. A., & Desnick, R. J. (1996) · Science · doi:10.1126/science.273.5279.1236 · Cited by 1,029
Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage.
Billinghurst, R. C., Dahlberg, L., Ionescu, M., Reiner, A., Bourne, R., Rorabeck, C., Mitchell, P., Hambor, J., Diekmann, O., Tschesche, H., et al. (1997) · Journal of Clinical Investigation · doi:10.1172/jci119316 · Cited by 968
Mitchell, P. G., Magna, H. A., Reeves, L. M., Lopresti-Morrow, L. L., Yocum, S. A., Rosner, P. J., Geoghegan, K. F., & Hambor, J. E. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118475 · Cited by 940
Biochemical Characterization of Human Collagenase-3
Knäuper, V., López-Otin, C., Smith, B., Knight, G., & Murphy, G. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.3.1544 · Cited by 881
Cathepsin K, but Not Cathepsins B, L, or S, Is Abundantly Expressed in Human Osteoclasts
Drake, F. H., Dodds, R. A., James, I. E., Connor, J. R., Debouck, C., Richardson, S., Lee-Rykaczewski, E., Coleman, L., Rieman, D., Barthlow, R., et al. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.21.12511 · Cited by 720
Biochemical and immunological characterization of the secreted forms of human neutrophil gelatinase.
Hibbs, M. S., Hasty, K. A., Seyer, J. M., Kang, A. H., & Mainardi, C. L. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)89580-1 · Cited by 613
Hollander, A. P., Heathfield, T. F., Webber, C., Iwata, Y., Bourne, R., Rorabeck, C., & Poole, A. R. (1994) · Journal of Clinical Investigation · doi:10.1172/jci117156 · Cited by 611
Self-citation(1988) · Annals of Internal Medicine · doi:10.7326/0003-4819-108-3-507_3 · Cited by 592
Cited by
Tjäderhane, L., Buzalaf, M. A. R., Carrilho, M., & Chaussain, C. (2015) · Caries Research · doi:10.1159/000363582 · Cited by 2,306
Impaired osteoclastic bone resorption leads to osteopetrosis in cathepsin-K-deficient mice
Saftig, P., Hunziker, E., Wehmeyer, O., Jones, S., Boyde, A., Rommerskirch, W., Moritz, J. D., Schu, P., & von Figura, K. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.23.13453 · Cited by 913
Sukhova, G. K., Shi, G. P., Simon, D. I., Chapman, H. A., & Libby, P. (1998) · Journal of Clinical Investigation · doi:10.1172/jci181 · Cited by 665
Cartilage in normal and osteoarthritis conditions
Martel-Pelletier, J., Boileau, C., Pelletier, J.-P., & Roughley, P. J. (2008) · Best Practice & Research Clinical Rheumatology · doi:10.1016/j.berh.2008.02.001 · Cited by 559
Choe, Y., Leonetti, F., Greenbaum, D. C., Lecaille, F., Bogyo, M., Brömme, D., Ellman, J. A., & Craik, C. S. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m513331200 · Cited by 425
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Metadata
- Authors
- KAFIENAH, W., BRÖMME, D., BUTTLE, D. J., CROUCHER, L. J., & HOLLANDER, A. P.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1998-05-01
- Volume
- 331
- Issue
- 3
- Pages
- 727-732
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- OpenAlex
- W1762318613
- Cited by
- 358
- References
- 38
- FWCI
- 5.22
- Type
- journal article
- Language
- en
- Open access
- green
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- KAFIENAH, W., BRÖMME, D., BUTTLE, D. J., CROUCHER, L. J., & HOLLANDER, A. P. (1998). Human cathepsin K cleaves native type I and II collagens at the N-terminal end of the triple helix. Biochemical Journal. https://doi.org/10.1042/bj3310727
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