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Cited by 152Amino Acid Enzymes and MetabolismCancer, Hypoxia, and MetabolismBiochemical and Molecular Research

The DOI matches its Crossref record; 28 of the 30 references it cites were found in a registry or an index.

Inhibition of glutaminase expression by antisense mRNA decreases growth and tumourigenicity of tumour cells

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LOBO, Carolina, RUIZ-BELLIDO, Miguel A., ALEDO, Juan C., MÁRQUEZ, Javier, NÚÑEZ DE CASTRO, Ignacio, ALONSO, Francisco J.·2000·Biochemical Journal
DOI 10.1042/bj3480257PMC PMC1221061
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Abstract

Phosphate-activated glutaminase has a critical role in tumours and rapidly dividing cells and its activity is correlated with malignancy. Ehrlich ascites tumour cells transfected with the pcDNA3 vector containing an antisense segment (0.28 kb) of rat kidney glutaminase showed impairment in the growth rate and plating efficiency, as well as a shortage in the glutaminase protein and activity. The C-terminal segment used is well conserved in all glutaminase sequences known. The transfected cells, named 0.28AS-2, displayed remarkable changes in their morphology compared with the parental cell line. The 0.28AS-2 cells also lost their tumourigenic capacity in vivo. Control mice developed an ascitic tumour, with a lifespan of 16±1 days, when inoculated with 107 cells/mouse; on the contrary, animals inoculated with transfected cells up to 2.5 times the cell numbers of control mice did not develop tumours and behaved as healthy animals. The ability to revert the transformed phenotype of antisense-transfected cells confirms the relevance of glutaminase in the transformation process and could provide new ways for the study of gene therapy.

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Research works printed but not linked: 2

  1. Murray, J. A. H. and Crockett, N. (1992) in Antisense RNA and DNA (Murray, H., ed.), pp. 1-49, Wiley-Liss, New York 22
  2. Gallagher, M. P., Marshall, R. D. and Wilson, R. (1989) Essays in Biochemistry, vol. 24 (Marshall, R. D. and Tipton, K. F., eds.), pp. 1-40, Academic Press, London

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Metadata

Authors
LOBO, C., RUIZ-BELLIDO, M. A., ALEDO, J. C., MÁRQUEZ, J., NÚÑEZ DE CASTRO, I., & ALONSO, F. J.
Affiliations
  • Universidad de Málaga (ES)
  • Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Campus de Teatinos, Universidad de Málaga, 29071 Málaga, Spain
ORCID
Venue
Biochemical Journal
Publisher
Portland Press Ltd.
Published
2000-05-23
Volume
348
Issue
2
Pages
257-261
ISSN
  • 0264-6021 (ISSN-L)
  • 1470-8728
DOI
10.1042/bj3480257
PMC
PMC1221061
OpenAlex
W1998424488
Cited by
151
References
30
Citations by year
2026: 2 · 2025: 4 · 2024: 2 · 2023: 13 · 2022: 3 · 2021: 3 · 2020: 3 · 2019: 9 · 2018: 6 · 2017: 5 · 2016: 7 · 2015: 10 · 2014: 9 · 2013: 8 · 2012: 8
FWCI
2.08
Type
journal article
Language
en
Open access
Locations
  • Biochemical Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Molecular Research
Keywords
glutaminase, antisense RNA, phosphate-activated glutaminase, Ehrlich ascites tumor cells, tumor growth inhibition, tumorigenicity, gene therapy, cell transformation, plating efficiency, cell morphology
MeSH terms
Animals, Carcinoma, Ehrlich Tumor, Cell Division, Glutaminase, Kidney, Kinetics, RNA, Messenger, Transfection, Tumor Cells, Cultured, Tumor Stem Cell Assay, Gene Expression Regulation, Enzymologic, RNA, Antisense, Mice, Rats

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