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Cited by 32Spine and Intervertebral Disc PathologySirtuins and Resveratrol in MedicineOsteoarthritis Treatment and Mechanisms

Nucleus pulposus cell senescence is alleviated by resveratrol through regulating the ROS/NF-κB pathway under high-magnitude compression

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Jiang, Yanhai, Dong, Guozhang, Song, Yeliang·2018·Bioscience Reports
DOI 10.1042/bsr20180670PMC PMC6435537
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Abstract

Mechanical overloading is a risk factor of disc degeneration. Studies have demonstrated that resveratrol helps to maintain the disc cell’s healthy biology. The present study aims to investigate whether resveratrol can suppress mechanical overloading-induced nucleus pulposus (NP) cell senescence in vitro and the potential mechanism. The isolated rat NP cells were seeded in the decalcified bone matrix (DBM) and cultured under non-compression (control) and compression (20% deformation, 1.0 Hz, 6 h/day) for 5 days using the mechanically active bioreactor. The resveratrol (30 and 60 μM) was added into the culture medium of the compression group to investigate its protective effects against the NP cell senescence. NP cell senescence was evaluated by cell proliferation, cell cycle, senescence-associated β-galactosidase (SA-β-Gal) activity, telomerase (TE) activity, and gene expression of the senescence markers (p16 and p53). Additionally, the reactive oxygen species (ROS) content and activity of the NF-κB pathway were also analyzed. Compared with the non-compression group, the high-magnitude compression significantly promoted NP cell senescence, increased ROS generation and activity of the NF-κB pathway. However, resveratrol partly attenuated NP cell senescence, decreased ROS generation and activity of the NF-κB pathway in a concentration-dependent manner under mechanical compression. Resveratrol can alleviate mechanical overloading-induced NP cell senescence through regulating the ROS/NF-κB pathway. The present study provides that resveratrol may be a potential drug for retarding mechanical overloading-induced NP cell senescence.

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Cited by

Sorted by citations

Authors
Jiang, Y., Dong, G., & Song, Y.
Affiliations
Qingdao University (CN)
Corresponding author
Song, Yeliang
ORCID
Venue
Bioscience Reports
Publisher
Portland Press Ltd.
Published
2018-07-06
Volume
38
Issue
4
ISSN
  • 0144-8463 (ISSN-L)
  • 1573-4935
DOI
10.1042/bsr20180670
PMC
PMC6435537
OpenAlex
W2805750621
Cited by
32
References
3
Citations by year
2026: 1 · 2025: 4 · 2024: 4 · 2023: 4 · 2022: 5 · 2021: 6 · 2020: 5 · 2019: 3
FWCI
2.66
Type
journal article
Language
en
Status
Retracted
Update notices
retraction (2024-08-22)
Open access
Licence
Locations
  • Bioscience Reports (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Spine and Intervertebral Disc Pathology
  • Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
Keywords
resveratrol, nucleus pulposus cells, cellular senescence, mechanical overload, reactive oxygen species, NF-κB signaling, intervertebral disc degeneration, telomerase activity, p16, p53, demineralized bone matrix
MeSH terms
Nucleus Pulposus, Resveratrol, Animals, Antioxidants, Cells, Cultured, Male, NF-kappa B, Weight-Bearing, Cellular Senescence, Rats, Sprague-Dawley, Reactive Oxygen Species

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