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Mitochondrial matrix reactive oxygen species production is very sensitive to mild uncoupling
Abstract
Mitochondria produce ROS (reactive oxygen species) as a by-product of aerobic respiration. Several studies in mammals and birds suggest that the most physiologically relevant ROS production is from complex I following reverse electron flow, and is highly sensitive to membrane potential. A study of Drosophila mitochondria respiring glycerol 3-phosphate revealed that membrane potential-sensitive ROS production from complex I following reverse electron flow was on the matrix side of the inner membrane. A 10 mV decrease in membrane potential was enough to abolish around 70% of the ROS produced by complex I under these conditions. Another important ROS generator in this model, glycerol-3-phosphate dehydrogenase, produced ROS mostly to the cytosolic side; this ROS production was totally insensitive to a small decrease in membrane potential (10 mV). Thus mild uncoupling may be particularly significant for ROS production from complex I on the matrix side of the mitochondrial inner membrane.
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Citations
Cited by
Calcium, ATP, and ROS: a mitochondrial love-hate triangle
Brookes, P. S., Yoon, Y., Robotham, J. L., Anders, M. W., & Sheu, S.-S. (2004) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00139.2004 · Cited by 2,575
Metabolic control through the PGC-1 family of transcription coactivators
Lin, J., Handschin, C., & Spiegelman, B. M. (2005) · Cell Metabolism · doi:10.1016/j.cmet.2005.05.004 · Cited by 2,130
“…PGC-1α and β stimulate the expression of UCP2 and UCP3 (Puigserver et al., 2001; St-Pierre et al., 2003; Wu et al., 1999), which dissipate the proton gradient and lower mitochondrial membrane potential, a process thought to greatly reduce ROS production by mitochondria (Miwa and Brand, 2003).”
BackgroundMitochondrial uncoupling, ROS generation and cardioprotection
Cadenas, S. (2018) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2018.05.019 · Cited by 591
Relation Between Mitochondrial Membrane Potential and ROS Formation
Suski, J. M., Lebiedzinska, M., Bonora, M., Pinton, P., Duszynski, J., & Wieckowski, M. R. (2012) · Methods in Molecular Biology · doi:10.1007/978-1-61779-382-0_12 · Cited by 522
Tahara, E. B., Navarete, F. D. T., & Kowaltowski, A. J. (2009) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2009.02.008 · Cited by 479
Metadata
- Authors
- Miwa, S., & Brand, M. D.
- Venue
- Biochemical Society Transactions
- Publisher
- Portland Press Ltd.
- Published
- 2003-12-01
- Volume
- 31
- Issue
- 6
- Pages
- 1300-1301
- ISSN
- 0300-5127 (ISSN-L)
- 1470-8752
- OpenAlex
- W2087282685
- Cited by
- 285
- References
- 0
- FWCI
- 3.86
- Type
- journal article
- Language
- en
- Open access
- closed
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- Miwa, S., & Brand, M. D. (2003). Mitochondrial matrix reactive oxygen species production is very sensitive to mild uncoupling. Biochemical Society Transactions. https://doi.org/10.1042/bst0311300
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