Epigenetic inactivation of PCDH10 in human prostate cancer cell lines
Abstract
PCDH10 (protocadherin‐10), a novel tumour suppressor gene, is down‐regulated in several human cancers due to hypermethylation of promoter CGIs (CpG islands). Here, we investigated the expression of PCDH10 in different normal adult tissues and in a panel of prostate cancer cell lines. PCDH10 was widely expressed in normal tissues with higher levels in the prostate. The expression of PCDH10 was markedly reduced or silenced in prostate cancer cell lines compared with normal adult prostate tissue. Decreased PCDH10 expression was correlated with the methylation status of the PCDH10 promoter. Furthermore, the DNA demethylating agent 5′‐azacytidin restored PCDH10 expression by suppressing PCDH10 promoter methylation in prostate cancer cell lines. Treatment with Trichostatin A alone had no significant effect on the expression of PCDH10 but enhanced the effect of 5′‐azacytidin. In conclusion, we found that the decreased PCDH10 expression in prostate cancer cells was associated with the aberrant methylation of PCDH10 promoter CGI. Our results may contribute to the understanding of the role of PCDH10 inactivation in the progression of prostate cancers.
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pcdh10a is included in the co-enrichment list of melanocytes and iridophores.
“Also included is the cell adhesion molecule pcdh10a, which is expressed by migrating zebrafish neural crest cells, and acts as a tumor suppressor in several cancers [61]-[63].”
pcdh10a is expressed by migrating zebrafish neural crest cells and acts as a tumor suppressor in several cancers.
“which is expressed by migrating zebrafish neural crest cells, and acts as a tumor suppressor in several cancers [61]-[63].”
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Higdon, C. W., Mitra, R. D., & Johnson, S. L. (2013) · PLoS ONE · doi:10.1371/journal.pone.0067801 · Cited by 125 · see citing works
- Authors
- Li, Z., Li, W., Xie, J., Wang, Y., Tang, A., Li, X., Ye, J., Gui, Y., & Cai, Z.
- Affiliations
- Shenzhen University (CN)
- Shantou University Medical College (CN)
- Peking University Shenzhen Hospital (CN)
- Corresponding author
- Li, Zesong
- Li, Wenjie
- Gui, Yaoting
- Cai, Zhiming
- Venue
- Cell Biology International
- Publisher
- Wiley
- Published
- 2011-07
- Volume
- 35
- Issue
- 7
- Pages
- 671-676
- ISSN
- 1065-6995 (ISSN-L)
- 1095-8355
- OpenAlex
- W2004800370
- Cited by
- 25
- References
- 0
- Citations by year
- 2024: 1 · 2023: 2 · 2021: 1 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 1 · 2014: 6 · 2013: 2 · 2012: 4
- FWCI
- 1.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell Biology International (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Prostate Cancer Treatment and Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer-related gene regulation
- Keywords
- PCDH10, prostate cancer, epigenetic silencing, promoter hypermethylation, CpG islands, tumor suppressor, azacitidine, DNA demethylation, trichostatin A, histone deacetylase, gene expression
- MeSH terms
- Protocadherins, Adult, Cytidine Monophosphate, Drug Synergism, Humans, Hydroxamic Acids, Male, Promoter Regions, Genetic, Prostatic Neoplasms, Blotting, Western, Cadherins, Gene Expression Regulation, Neoplastic, Polymerase Chain Reaction, CpG Islands, DNA Methylation, Epigenesis, Genetic, Cell Line, Tumor
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