Circadian variation in vascular tone and endothelial cell function in normal males
Abstract
The existence of circadian rhythms in the time of onset of acute cardiovascular events has been described previously. This report describes the circadian variation in endothelial cell products, such as nitric oxide (NO) and endothelin-1 (ET-1) levels, and endothelium-dependent and -independent vasodilation in normal males. Plasma ET-1 and NO were measured every 4 h in nine subjects (20-41 years old) over a 24 h period. Endothelium-dependent and -independent vascular responses were measured in the forearm skin every 4 h using laser Doppler imaging after iontophoresis of increasing doses of acetylcholine (ACh) and sodium nitroprusside respectively. A statistically significant circadian variation was demonstrated for the mean ACh response (P=0.0001, ANOVA). The peak response [in arbitrary perfusion units (AU)] occurred at 16.00 hours (8.90+/-1.91 AU) and the lowest response at 08.00 hours (4.57+/-0.66 AU). A significant circadian variation was also seen for the highest dose of sodium nitroprusside (P=0.036, ANOVA), the peak occurred at 16.00 hours (3.97+/-1.80 AU), and the lowest at 04.00 hours (2.62+/-0.58 AU) and 08.00 hours (2.58+/-1.16 AU). There was a significant circadian variation in the ET-1 levels (P=0.04) with two peaks, one at 20.00 hours (0.80+/-0.28 pg/ml) and the other at 08.00 hours (0.84+/-0.15 pg/ml). The lowest value occurred at 16.00 hours (0.61+/-0.24 pg/ml). There was also a borderline trend for a circadian variation in NO levels (P=0.06), with higher levels at 20.00 hours (15.53+/-8.42 micromol/l), and low levels at 04.00 hours (10.87+/-4.70 micromol/l) and 08.00 hours (9.82+/-3.15 micromol/l). ACh responses were significantly correlated with ET-1 (r=-0.3, P=0.02) and NO (r=0.30, P=0.02) levels. Our findings suggest that endothelial activity has a circadian variation with attenuation in the morning. These circadian variations in endothelial activity might play an important role in the occurrence of acute cardiovascular events at this time, which are precipitated through the interplay between ET-1, NO and vascular function.
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In humans, vascular endothelial function is relatively impaired in the morning, and therefore potentially implicated in the morning increase in adverse cardiovascular events.
“In humans, vascular endothelial function is relatively impaired in the morning (6, 123-126), and therefore potentially implicated in the morning increase in adverse CV events (127).”
From Role of the circadian system in cardiovascular disease (2018) · cites this paper as [124]
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References 22 · Cited by 110
- Authors
- ELHERIK, K., KHAN, F., MCLAREN, M., KENNEDY, G., & BELCH, J. J. F.
- Affiliations
- Ninewells Hospital (GB)
- Venue
- Clinical Science
- Publisher
- Portland Press Ltd.
- Published
- 2002-04-18
- Volume
- 102
- Issue
- 5
- Pages
- 547-552
- ISSN
- 0143-5221 (ISSN-L)
- 1470-8736
- OpenAlex
- W2011791979
- Cited by
- 110
- References
- 22
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 5 · 2023: 4 · 2021: 4 · 2020: 4 · 2019: 5 · 2018: 2 · 2017: 2 · 2016: 6 · 2015: 6 · 2014: 5 · 2013: 4 · 2012: 4
- FWCI
- 3.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinical Science (publishedVersion)
- PubMed (publishedVersion)
- Discovery Research Portal (University of Dundee) (submittedVersion)
- Funders
- Tenovus
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Health and Disease Prevention
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Eicosanoids and Hypertension Pharmacology
- Keywords
- circadian rhythm, vascular tone, endothelial function, nitric oxide, endothelin-1, endothelium-dependent vasodilation, endothelium-independent relaxation, acetylcholine, sodium nitroprusside, plasma nitric oxide, plasma endothelin-1 levels, time of day, healthy men, acute cardiovascular events, laser Doppler imaging
- MeSH terms
- Acetylcholine, Adult, Analysis of Variance, Circadian Rhythm, Endothelium, Vascular, Humans, Male, Nitric Oxide, Nitroprusside, Skin, Vasodilation, Vasodilator Agents, Endothelin-1
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