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Immunological characterization of Echinococcus granulosus cyclophilin, an allergen reactive with IgE and IgG4 from patients with cystic echinococcosis

ORTONA, E, VACCARI, S, MARGUTTI, P, DELUNARDO, F, RIGANÒ, R, PROFUMO, E, BUTTARI, B, RASOOL, O, TEGGI, A, SIRACUSANO, A·2002·Clinical and Experimental Immunology
DOI 10.1046/j.1365-2249.2002.01807.x
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Abstract

SUMMARY: By immunological screening of a cDNA library derived from protoscoleces of Echinococcus granulosus with IgE from patients with cystic echinococcosis (CE) and allergic manifestations, we isolated a protein identical to E. granulosus cyclophilin. The protein, named EA21, has close homology with Malassezia furfur cyclophilin allergen (Mal f 6) and with human cyclophilin. Using immunoblotting (IB) with a polyclonal antibody specific to EA21, we identified E. granulosus cyclophilin both in protoscoleces and in sheep hydatid fluid. Of the 58 sera from patients with CE, 29 (50%) were IgE positive to EA21, whereas, despite the high sequence homology, none were IgE positive to Mal f 6 or human cyclophilin. Only 26 of the 58 patients (45%) had IgG specific to EA21, whereas all patients (100%) had IgG specific to Mal f 6 and human cyclophilin. IB analysis showed that serum IgE-binding reactivity to EA21 differed significantly in patients with and without allergic reactions (20 of 25, 80%versus nine of 33, 27%; P < 10–4). Conversely, five of the 25 patients who had CE-related allergic manifestations (20%) and 21 of the 33 who did not (63%) had specific IgG4 (P = 10–3) and total IgG to EA21. EA21 induced a proliferative response in 15 of 19 (79%) patients’ PBMC regardless of the allergic manifestations, but it induced no IL-4 production. Overall, these findings suggest that E. granulosus cyclophilin is a conserved, constitutive, parasite protein that does not cross-react with cyclophilins from other organisms and is involved in the allergic symptoms related to CE.

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Research works printed but not linked: 26

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Metadata

Authors
ORTONA, E., VACCARI, S., MARGUTTI, P., DELUNARDO, F., RIGANÒ, R., PROFUMO, E., BUTTARI, B., RASOOL, O., TEGGI, A., & SIRACUSANO, A.
Venue
Clinical and Experimental Immunology
Publisher
Oxford University Press (OUP)
Published
2002-04
Volume
128
Issue
1
Pages
124-130
ISSN
  • 0009-9104
  • 1365-2249
DOI
10.1046/j.1365-2249.2002.01807.x
Cited by
45
References
0
Language
en
Licence
academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)

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CoChat. (2026). Citation check: Immunological characterization of Echinococcus granulosus cyclophilin, an allergen reactive with IgE and IgG4 from patients with cystic echinococcosis. https://app.cochat.ai/papers/10.1046/j.1365-2249.2002.01807.x
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ORTONA, E., VACCARI, S., MARGUTTI, P., DELUNARDO, F., RIGANÒ, R., PROFUMO, E., BUTTARI, B., RASOOL, O., TEGGI, A., & SIRACUSANO, A. (2002). Immunological characterization of Echinococcus granulosus cyclophilin, an allergen reactive with IgE and IgG4 from patients with cystic echinococcosis. Clinical and Experimental Immunology. https://doi.org/10.1046/j.1365-2249.2002.01807.x