Inflammatory cytokine production by immunological and foreign body multinucleated giant cells
Open accessAbstract
Multinucleated giant cells (MGC) are a common feature of granulomas. The mechanism of their formation has been studied extensively, but their function has not been completely characterized. A new method for the in vivo production of MGC was developed involving subcutaneous injection of microscopic nitrocellulose particles with adsorbed mycobacterial antigens into the footpads of sensitized BALB/c mice (immune [I]-MGC), or by nitrocellulose administration to non-sensitized mice (foreign body [FB]-MGC). The development of granulomas with a highly enriched MGC population was observed 2 weeks after the nitrocellulose injection. MGC were larger with a greater number of nuclei in I-MGC than in FB-MGC. From days 7-28 after nitrocellulose administration, the production of interleukin-1alpha (IL-1alpha) and tumour necrosis factor-alpha (TNF-alpha) was demonstrated in both MGC types by in situ reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. After 2 months, the MGC had ceased production of IL-1alpha and TNF-alpha, but the expression of transforming growth factor-beta (TGF-beta) was very high, occurring together with extensive fibrosis. These results suggest that MGC are an active source of inflammatory cytokines, which can contribute to the initiation, maintenance and down-regulation of granulomatous inflammation induced by immunological and inert substances.
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FBGCs, derived from a murine model injected with nitrocellulose particles, showed production of IL-1α and TNF-α only during the first month of the foreign body reaction and subsequently initiated the production of TGF-β, but did not show the production of macrophage inflammatory proteins (MIP).
“FBGCs, derived from a murine model injected with nitrocellulose particles, showed production of IL-1α and TNF-α only during the first month of the foreign body reaction and subsequently initiated the production of transforming growth factor-β (TGF-β), but did not show the production of macrophage inflammatory proteins (MIP).[81]”
From Foreign body reaction to biomaterials (2008) · cites this paper as [81]
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References 27 · Cited by 113
- Authors
- Hernandez‐Pando, R., Bornstein, Q. L., Aguilar Leon, D., Orozco, E. H., Madrigal, V. K., & Martinez Cordero, E.
- Affiliations
- Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán (MX)
- Instituto Nacional de Enfermedades Respiratorias (MX)
- Venue
- Immunology
- Publisher
- Wiley
- Published
- 2000-07
- Volume
- 100
- Issue
- 3
- Pages
- 352-358
- ISSN
- 0019-2805 (ISSN-L)
- 1365-2567
- PMC
- PMC2327020
- OpenAlex
- W1969229361
- Cited by
- 113
- References
- 27
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 3 · 2023: 8 · 2022: 6 · 2021: 6 · 2020: 6 · 2019: 3 · 2018: 4 · 2017: 1 · 2016: 6 · 2015: 3 · 2014: 7 · 2013: 7 · 2012: 2
- FWCI
- 1.04
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Immunology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Health Sciences › Medicine › Physiology › Sarcoidosis and Beryllium Toxicity Research
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- multinucleated giant cells, granuloma, inflammatory cytokines, interleukin-1 alpha, TNF-α, TGF-beta, mycobacterial antigens, foreign body granuloma, fibrosis, granulomatous inflammation
- MeSH terms
- Animals, Collodion, Granuloma, Immunoenzyme Techniques, Male, Mice, Inbred BALB C, Microscopy, Electron, Giant Cells, Giant Cells, Foreign-Body, Gene Expression, Cytokines, Microscopy, Immunoelectron, Reverse Transcriptase Polymerase Chain Reaction, Mice
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