The DOI matches its Crossref record.
Development and use of 16S rRNA gene targeted PCR primers for the identification of Escherichia coli cells in water
Abstract
The primary sequences of the V3 and V6 regions of the 16S rRNA gene of pathogenic and non-pathogenic strains of Escherichia coli were determined and compared with those obtained for a number of reference strains which belong to the family Enterobacteriaceae. Three oligonucleotide primers 16E1, 16E2 and 16E3 were designed and used in the polymerase chain reaction to identify specifically all E. coli isolates. When 16E1, 16E2 and 16E3 were used as primers for the identification of E. coli cells present in tap, underground and pond waters, as low as 1 cfu 100 ml-1 of water could be detected if an 8 h pre-culture step was performed prior to the PCR reaction.
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“According to Tsen et al. (1998) report, our isolate S2-9 is identified as non-pathogenic E. coli from water samples.”
From Isolation and identification of high fluoride resistant bacteria from water samples of Dindigul district, Tamil Nadu, South India (2021) · cites this paper as Tsen et al. (1998)
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Chellaiah, E. R., Ravi, P., & Uthandakalaipandian, R. (2021) · Current Research in Microbial Sciences · doi:10.1016/j.crmicr.2021.100038 · Cited by 17
Metadata
- Authors
- Tsen, H. Y., Lin, C. K., & Chi, W. R.
- Affiliations
- Department of Food Science, National Chung‐Hsing University,
- Department of Food Science and Nutrition, Hung Kung Institute of Technology, Taichung, Taiwan, Republic of China
- Venue
- Journal of Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 1998-09
- Volume
- 85
- Issue
- 3
- Pages
- 554-560
- ISSN
- 1364-5072
- 1365-2672
- Cited by
- 92
- References
- 0
- Language
- en
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- CoChat. (2026). Citation check: Development and use of 16S rRNA gene targeted PCR primers for the identification of Escherichia coli cells in water. https://app.cochat.ai/papers/10.1046/j.1365-2672.1998.853535.x
- The paper
- Tsen, H. Y., Lin, C. K., & Chi, W. R. (1998). Development and use of 16S rRNA gene targeted PCR primers for the identification of Escherichia coli cells in water. Journal of Applied Microbiology. https://doi.org/10.1046/j.1365-2672.1998.853535.x
