Evidence for Oestrogen Receptor α‐Immunoreactivity in Gonadotrophin‐Releasing Hormone‐Expressing Neurones
Abstract
Having used the cingulate cortex to demonstrate the validity of our methods for detecting hitherto unrecognized oestrogen receptor alpha (ERalpha)-immunoreactive neurones, we have now employed immunoprecipitation and double-label immunohistochemistry to investigate whether the ERalpha protein is present in gonadotrophin-releasing hormone (GnRH)-containing cells. The immortalized GnRH cell line GT1-7 and GnRH neurones within the rat preoptic area were found to possess ERalpha-immunoreactivity (ERalpha-IR). These observations indicate that oestrogen may regulate the synthesis and release of GnRH by direct actions on GnRH neurones.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Some immunohistochemistry studies in rat brain have detected ERβ in GnRH neurons.
“Some studies using immunohistochemistry in rat brain have indeed shown the presence of ERβ in GnRH neuron (Butler, Sjoberg, & Coen, 1999; Hrabovszky et al., 2007; Hrabovszky et al., 2001; Kallo, Butler, Barkovics-Kallo, Goubillon, & Coen, 2001; Navarro et al., 2003; Roy, Angelini, & Belsham, 1999).”
From Hypothalamic–pituitary–adrenal and hypothalamic–pituitary–gonadal axes: sex differences in regulation of stress responsivity (2017) · cites this paper as Butler, Sjoberg, & Coen, 1999
Cites
Absence of oestradiol concentration in cell nuclei of LHRH-immunoreactive neurones
Shivers, B. D., Harlan, R. E., Morrell, J. I., & Pfaff, D. W. (1983) · Nature · doi:10.1038/304345a0 · Cited by 531 · see citing works
Herbison, A. E., & Theodosis, D. T. (1992) · Neuroscience · doi:10.1016/0306-4522(92)90423-y · Cited by 283 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Oyola, M. G., & Handa, R. J. (2017) · Stress · doi:10.1080/10253890.2017.1369523 · Cited by 710 · see citing works
Identification and characterization of a gonadotropin-inhibitory system in the brains of mammals
Kriegsfeld, L. J., Mei, D. F., Bentley, G. E., Ubuka, T., Mason, A. O., Inoue, K., Ukena, K., Tsutsui, K., & Silver, R. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0511003103 · Cited by 552 · see citing works
Estrogenic Regulation of the GnRH Neuron
Radovick, S., Levine, J. E., & Wolfe, A. (2012) · Frontiers in Endocrinology · doi:10.3389/fendo.2012.00052 · Cited by 102 · see citing works
Helena, C. V. V., de Oliveira Poletini, M., Sanvitto, G. L., Hayashi, S., Franci, C. R., & Anselmo-Franci, J. A. (2006) · Journal of Endocrinology · doi:10.1677/joe.1.06268 · Cited by 53 · see citing works
- Authors
- Butler, Sjöberg, & Coen
- Affiliations
- King's College London (GB)
- Venue
- Journal of Neuroendocrinology
- Publisher
- Wiley
- Published
- 1999-05
- Volume
- 11
- Issue
- 5
- Pages
- 331-335
- ISSN
- 0953-8194 (ISSN-L)
- 1365-2826
- OpenAlex
- W1503554255
- Cited by
- 125
- References
- 2
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 1 · 2023: 1 · 2020: 1 · 2017: 6 · 2016: 4 · 2015: 2 · 2014: 3 · 2012: 3
- FWCI
- 18.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neuroendocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Keywords
- estrogen receptor alpha, gonadotropin-releasing hormone, preoptic area, rats
- MeSH terms
- Acrolein, Animals, Antibody Specificity, Cell Extracts, Cell Line, Cell Nucleus, Female, Formaldehyde, Glutaral, Immunohistochemistry, Gonadotropin-Releasing Hormone, Molecular Weight, Neurons, Ovariectomy, Polymers, Precipitin Tests, Preoptic Area, Receptors, Estrogen, Reproducibility of Results, Artifacts, Estrogen Receptor alpha, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
