The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
Identification of csrR/csrS, a genetic locus that regulates hyaluronic acid capsule synthesis in group A Streptococcus
Abstract
The hyaluronic acid capsule of group A Streptococcus (GAS) is an important virulence factor, but little is known about mechanisms that regulate capsule expression. Transposon Tn 916 mutagenesis of the poorly encapsulated M‐type 3 GAS strain DLS003 produced a transconjugant that exhibited a mucoid colony morphology, reflecting increased hyaluronic acid capsule production. Analysis of chromosomal DNA sequence immediately downstream of the transposon insertion identified two open reading frames, designated csrR and csrS, which exhibited sequence similarity to bacterial two‐component regulatory systems. We constructed an in‐frame deletion mutation within csrR, which encodes the putative response component. Replacement of the native csrR gene in the DLS003 chromosome with the mutant allele resulted in a sixfold increase in capsule production and a corresponding increase in transcription of the has operon, which contains the essential genes for hyaluronic acid synthesis. Increased capsule production by the csrR mutant strain was associated with enhanced resistance to complement‐mediated opsonophagocytic killing in vitro and with a 500‐fold increase in virulence in mice. These results establish CsrR as a negative regulator of hyaluronic acid capsule synthesis and suggest that it is part of a two‐component regulatory system that influences capsule expression and virulence.
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Basic local alignment search tool
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990) · Journal of Molecular Biology · doi:10.1006/jmbi.1990.9999 · Cited by 98,669
A SIMPLE METHOD OF ESTIMATING FIFTY PER CENT ENDPOINTS12
REED, L. J., & MUENCH, H. (1938) · American Journal of Epidemiology · doi:10.1093/oxfordjournals.aje.a118408 · Cited by 19,926
Yanisch-Perron, C., Vieira, J., & Messing, J. (1985) · Gene · doi:10.1016/0378-1119(85)90120-9 · Cited by 15,041
A simple method of estimating 50 per cent end points
Muench, H. (1938) · Medical Entomology and Zoology · Cited by 8,376
Dudley, R. K. (1988) · FEBS Letters · doi:10.1016/0014-5793(88)80264-3 · Cited by 5,945
Protein phosphorylation and regulation of adaptive responses in bacteria
Stock, J., Ninfa, A. J., & Stock, A. (1989) · Microbiological Reviews · doi:10.1128/mmbr.53.4.450-490.1989 · Cited by 1,707
COMMUNICATION MODULES IN BACTERIAL SIGNALING PROTEINS
Parkinson, J. S., & Kofoid, E. C. (1992) · Annual Review of Genetics · doi:10.1146/annurev.ge.26.120192.000443 · Cited by 1,450
Stevens, D. L., Tanner, M. H., Winship, J., Swarts, R., Ries, K. M., Schlievert, P. M., & Kaplan, E. (1989) · New England Journal of Medicine · doi:10.1056/nejm198907063210101 · Cited by 1,216
Signal transduction schemes of bacteria
Parkinson, J. S. (1993) · Cell · doi:10.1016/0092-8674(93)90267-t · Cited by 772
Invasive Group A Streptococcal Infections in Ontario, Canada
Davies, H. D., McGeer, A., Schwartz, B., Green, K., Cann, D., Simor, A. E., & Low, D. E. (1996) · New England Journal of Medicine · doi:10.1056/nejm199608223350803 · Cited by 736
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Disease Manifestations and Pathogenic Mechanisms of Group A Streptococcus
Walker, M. J., Barnett, T. C., McArthur, J. D., Cole, J. N., Gillen, C. M., Henningham, A., Sriprakash, K. S., Sanderson-Smith, M. L., & Nizet, V. (2014) · Clinical Microbiology Reviews · doi:10.1128/cmr.00101-13 · Cited by 908
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Nobbs, A. H., Lamont, R. J., & Jenkinson, H. F. (2009) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00014-09 · Cited by 657
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Glaser, P., Rusniok, C., Buchrieser, C., Chevalier, F., Frangeul, L., Msadek, T., Zouine, M., Couvé, E., Lalioui, L., Poyart, C., et al. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.03126.x · Cited by 476
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Metadata
- Authors
- Levin, J. C., & Wessels, M. R.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 1998-10
- Volume
- 30
- Issue
- 1
- Pages
- 209-219
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2025828314
- Cited by
- 274
- References
- 52
- FWCI
- 15.72
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Identification of csrR/csrS, a genetic locus that regulates hyaluronic acid capsule synthesis in group A Streptococcus. https://app.cochat.ai/papers/10.1046/j.1365-2958.1998.01057.x
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- Levin, J. C., & Wessels, M. R. (1998). Identification of csrR/csrS, a genetic locus that regulates hyaluronic acid capsule synthesis in group A Streptococcus. Molecular Microbiology. https://doi.org/10.1046/j.1365-2958.1998.01057.x
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