The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
High‐efficiency cloning of Arabidopsis full‐length cDNA by biotinylated CAP trapper
Open accessAbstract
Summary: Full‐length cDNAs are essential for functional analysis of plant genes. We constructed high‐content, full‐length cDNA libraries from Arabidopsis thaliana plants based on chemical introduction of a biotin group into the diol residue of the CAP structure of eukaryotic mRNA, followed by RNase I treatment, to select full‐length cDNA. More than 90% of the total clones obtained were of full length; recombinant clones were obtained with high efficiency (2.2 × 10 6/9 μg starting mRNA). Sequence analysis of 111 randomly picked clones indicated that 32 isolated cDNA groups were derived from novel genes in the A. thaliana genome.
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Cites
Stockinger, E. J., Gilmour, S. J., & Thomashow, M. F. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.3.1035 · Cited by 1,807
Cited by
Seki, M., Narusaka, M., Ishida, J., Nanjo, T., Fujita, M., Oono, Y., Kamiya, A., Nakajima, M., Enju, A., Sakurai, T., et al. (2002) · The Plant Journal · doi:10.1046/j.1365-313x.2002.01359.x · Cited by 1,924
Self-citationWang, J., Ding, Y., Wang, J., Hillmer, S., Miao, Y., Lo, S. W., Wang, X., Robinson, D. G., & Jiang, L. (2011) · The Plant Cell · doi:10.1105/tpc.110.080697 · Cited by 357
Expressed sequence tags: alternative or complement to whole genome sequences?
Rudd, S. (2003) · Trends in Plant Science · doi:10.1016/s1360-1385(03)00131-6 · Cited by 296
Regulation of adaptive growth decisions via phosphorylation of the TRAPPII complex in Arabidopsis
(2024) · doi:10.1083/jcb.202311125 · Cited by 6
Metadata
- Authors
- Seki, M., Carninci, P., Nishiyama, Y., Hayashizaki, Y., & Shinozaki, K.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 1998-09
- Volume
- 15
- Issue
- 5
- Pages
- 707-720
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2049878158
- Cited by
- 218
- References
- 1
- FWCI
- 1.58
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Seki, M., Carninci, P., Nishiyama, Y., Hayashizaki, Y., & Shinozaki, K. (1998). High‐efficiency cloning of Arabidopsis full‐length cDNA by biotinylated CAP trapper. The Plant Journal. https://doi.org/10.1046/j.1365-313x.1998.00237.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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