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Cited by 417

The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.

The glutathione‐deficient, cadmium‐sensitive mutant,cad2–1, ofArabidopsis thalianais deficient in γ‐glutamylcysteine synthetase

Cobbett, Christopher S., May, Mike J., Howden, Ross, Rolls, Barbara·1998·The Plant Journal
DOI 10.1046/j.1365-313x.1998.00262.x
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Abstract

Summary: This paper reports that the glutathione (GSH)‐deficient mutant, cad2–1 , of Arabidopsis is deficient in the first enzyme in the pathway of GSH biosynthesis, γ‐glutamylcysteine synthetase (GCS). The mutant accumulates a substrate of GCS, cysteine, and is deficient in the product, γ‐glutamylcysteine. In vitro enzyme assays showed that the cad2–1 mutant has 40% of wild‐type levels of GCS activity but is unchanged in the activity of the second enzyme in the pathway, GSH synthetase. The CAD2 locus maps to chromosome 4 and is tightly linked to a gene, GSHA , identified by a previously isolated cDNA. A genomic clone of GSHA complements both the phenotypic and biochemical deficiencies of the cad2–1 mutant. The nucleotide sequence of the gene has been determined and, in the mutant, this gene contains a 6 bp deletion within an exon. These data demonstrate that the CAD2 gene encodes GCS. The cad2–1 mutation is close to the conserved cysteine which is believed to bind the substrate glutamate and the specific inhibitor L‐buthionine‐[S,R] sulfoximine (BSO). Both root growth and GCS activity of the cad2–1 mutant was less sensitive than the wild‐type to inhibition by BSO, indicating that the mutation may alter the affinity of the inhibitor binding site.

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Metadata

Authors
Cobbett, C. S., May, M. J., Howden, R., & Rolls, B.
Venue
The Plant Journal
Publisher
Wiley
Published
1998-10
Volume
16
Issue
1
Pages
73-78
ISSN
  • 0960-7412 (ISSN-L)
  • 1365-313X
DOI
10.1046/j.1365-313x.1998.00262.x
OpenAlex
W2138049951
Cited by
417
References
24
FWCI
2.25
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: The glutathione‐deficient, cadmium‐sensitive mutant,cad2–1, ofArabidopsis thalianais deficient in γ‐glutamylcysteine synthetase. https://app.cochat.ai/papers/10.1046/j.1365-313x.1998.00262.x
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Cobbett, C. S., May, M. J., Howden, R., & Rolls, B. (1998). The glutathione‐deficient, cadmium‐sensitive mutant,cad2–1, ofArabidopsis thalianais deficient in γ‐glutamylcysteine synthetase. The Plant Journal. https://doi.org/10.1046/j.1365-313x.1998.00262.x

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.