The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Cytokinin oxidase from Zea mays: purification, cDNA cloning and expression in moss protoplasts
Open accessAbstract
Summary: Cytokinins are degraded by cytokinin oxidases (CKOs) which catalyse cleavage of the N 6‐(isopent‐2‐enyl)‐side chain resulting in formation of adenine‐type compounds. CKO activity has been recorded in many plants and is thought to play a key role in controlling cytokinin levels in plants. Several partially purified CKOs have been characterised but no genes have been isolated yet. CKO activity is known to be inhibited by phenylureas, cytokinin agonists. We used 1‐(2‐azido‐6‐chloropyrid‐4‐yl)‐3‐(4‐[3 H])phenylurea ([3 H]‐azidoCPPU) to photolabel a glycosylated CKO from maize kernels. This enabled us to purify the enzyme. Peptide sequences were determined and the corresponding cDNA was cloned. The deduced amino acid sequence shares homology domains with FAD‐dependent oxidases. An original assay based on transient expression of the enzyme in moss protoplasts allowed the functionality of the recombinant enzyme to be demonstrated.
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Cites
Faiss, M., Zalubìlová, J., Strnad, M., & Schmülling, T. (1997) · The Plant Journal · doi:10.1046/j.1365-313x.1997.12020401.x · Cited by 305
Cited by
Werner, T., Motyka, V., Laucou, V., Smets, R., Van Onckelen, H., & Schmülling, T. (2003) · The Plant Cell · doi:10.1105/tpc.014928 · Cited by 1,527
C YTOKININ M ETABOLISM AND A CTION
Mok, D. W., & Mok, M. C. (2001) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.52.1.89 · Cited by 1,292
Regulation of plant growth by cytokinin
Werner, T., Motyka, V., Strnad, M., & Schmülling, T. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.171304098 · Cited by 1,133
Schmülling, T., Werner, T., Riefler, M., Krupková, E., & Bartrina y Manns, I. (2003) · Journal of Plant Research · doi:10.1007/s10265-003-0096-4 · Cited by 441
Evolution of cytokinin biosynthesis and degradation
Frebort, I., Kowalska, M., Hluska, T., Frebortova, J., & Galuszka, P. (2011) · Journal of Experimental Botany · doi:10.1093/jxb/err004 · Cited by 422
Metadata
- Authors
- Houba‐Hérin, N., Pethe, C., D’Alayer, J., & Laloue, M.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 1999-03
- Volume
- 17
- Issue
- 6
- Pages
- 615-626
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2030356145
- Cited by
- 191
- References
- 1
- FWCI
- 13.81
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Houba‐Hérin, N., Pethe, C., D’Alayer, J., & Laloue, M. (1999). Cytokinin oxidase from Zea mays: purification, cDNA cloning and expression in moss protoplasts. The Plant Journal. https://doi.org/10.1046/j.1365-313x.1999.00408.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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