The GRAS gene family in Arabidopsis: sequence characterization and basic expression analysis of the SCARECROW‐LIKE genes
Open accessAbstract
Summary: Mutations at the SCARECROW (SCR) locus in Arabidopsis thaliana result in defective radial patterning in the root and shoot. The SCR gene product contains sequences which suggest that it is a transcription factor. A number of Arabidopsis Expressed Sequence Tags (ESTs) have been identified that encode gene products bearing remarkable similarity to SCR throughout their carboxyl‐termini, indicating that SCR is the prototype of a novel gene family. These ESTs have been designated SCARECROW‐LIKE (SCL). The gene products of the GIBBERELLIN‐INSENSITIVE (GAI) and the REPRESSOR of ga1–3 (RGA) loci show high structural and sequence similarity to SCR and the SCLs. Sequence analysis of the products of the GRAS (GAI, RGA, SCR) gene family indicates that they share a variable amino‐terminus and a highly conserved carboxyl‐terminus that contains five recognizable motifs. The SCLs have distinct patterns of expression, but all of those analyzed show expression in the root. One of them, SCL3, has a tissue‐specific pattern of expression in the root similar to SCR. The importance of the GRAS gene family in plant biology has been established by the functional analyses of SCR, GAI and RGA.
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LAS is a member of the GRAS family of putative transcriptional regulators.
“The LAS protein is a member of the GRAS family of putative transcriptional regulators (Pysh et al. 1999).”
From Molecular analysis of the <i>LATERAL SUPPRESSOR</i> gene in <i>Arabidopsis</i> reveals a conserved control mechanism for axillary meristem formation (2003) · cites this paper as Pysh et al. 1999
The Evolutionary relationships section reports that tomato Lateral suppressor (Ls) is a GRAS-family member, and Definition of the GRAS family describes GRAS proteins as putative transcriptional regulators. The paper does not identify a protein named LAS as a member of the family, so the LAS-specific part of the claim is not established by this copy. (LLM, conf=0.82)
Experimental — accuracy not yet measuredThe characteristic sequence motifs of the GRAS family, like the VHIID domain, the two leucine heptad repeats, and a SAW domain, were identified in LAS.
“The characteristic sequence motifs of this protein family, like the VHIID domain, the two leucine heptad repeats, and a so-called SAW domain (Pysh et al. 1999), were also identified in LAS.”
From Molecular analysis of the <i>LATERAL SUPPRESSOR</i> gene in <i>Arabidopsis</i> reveals a conserved control mechanism for axillary meristem formation (2003) · cites this paper as Pysh et al. 1999
The LAS protein contains all the sequence motifs described for members of the GRAS family, except for the DELLA domain found in GAI, RGA, and a few other members of this gene family.
“The LAS protein contains all the sequence motifs described for members of the GRAS family (Pysh et al. 1999), except for the DELLA domain that was found in GAI, RGA, and a few other members of this gene family.”
From Molecular analysis of the <i>LATERAL SUPPRESSOR</i> gene in <i>Arabidopsis</i> reveals a conserved control mechanism for axillary meristem formation (2003) · cites this paper as Pysh et al. 1999
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Cites
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“The LAS protein is a member of the GRAS family of putative transcriptional regulators (Pysh et al. 1999).”
BackgroundInfluentialMechanisms regulating SHORT-ROOT intercellular movement.
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- Authors
- Pysh, L. D., Wysocka‐Diller, J. W., Camilleri, C., Bouchez, D., & Benfey, P. N.
- Affiliations
- New York University (US)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Corresponding author
- Benfey, Philip N.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 1999-04
- Volume
- 18
- Issue
- 1
- Pages
- 111-119
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2102612799
- Cited by
- 698
- References
- 3
- Citations by year
- 2026: 28 · 2025: 37 · 2024: 28 · 2023: 23 · 2022: 46 · 2021: 50 · 2020: 24 · 2019: 20 · 2018: 18 · 2017: 32 · 2016: 26 · 2015: 41 · 2014: 22 · 2013: 23 · 2012: 38
- FWCI
- 25.28
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Plant Journal (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- National Science Foundation
- National Institutes of Health
- National Institute of General Medical Sciences (R01-GM43778, R01GM043778)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Keywords
- GRAS gene family, scarecrow, Arabidopsis thaliana, transcription factors, radial patterning, root development, expressed sequence tags, gene expression patterns
- MeSH terms
- Amino Acid Sequence, Multigene Family, Molecular Sequence Data, Mutation, Gene Expression, Conserved Sequence, Genes, Plant, Arabidopsis, Sequence Homology, Amino Acid, In Situ Hybridization, Evolution, Molecular, Arabidopsis Proteins, Co-Repressor Proteins
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