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Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium
Open accessAbstract
Summary: Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T2 generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large‐scale insertional mutagenesis. T‐DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes.
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Citations
Cited by
Wroblewski, T., Tomczak, A., & Michelmore, R. (2005) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2005.00123.x · Cited by 558
Agrobacterium-Mediated Plant Transformation: Biology and Applications
Hwang, H.-H., Yu, M., & Lai, E.-M. (2017) · The Arabidopsis Book · doi:10.1199/tab.0186 · Cited by 312
Achievements and Challenges in Legume Breeding for Pest and Disease Resistance
Rubiales, D., Fondevilla, S., Chen, W., Gentzbittel, L., Higgins, T. J. V., Castillejo, M. A., Singh, K. B., & Rispail, N. (2015) · Critical Reviews in Plant Sciences · doi:10.1080/07352689.2014.898445 · Cited by 253
Transformation-induced mutations in transgenic plants: analysis and biosafety implications
Wilson, A. K., Latham, J. R., & Steinbrecher, R. A. (2006) · Biotechnology and Genetic Engineering Reviews · doi:10.1080/02648725.2006.10648085 · Cited by 96
Metadata
- Authors
- Trieu, A. T., Burleigh, S. H., Kardailsky, I. V., Maldonado‐Mendoza, I. E., Versaw, W. K., Blaylock, L. A., Shin, H., Chiou, T., Katagi, H., Dewbre, G. R., Weigel, D., & Harrison, M. J.
- Venue
- The Plant Journal
- Publisher
- Wiley
- Published
- 2000-06
- Volume
- 22
- Issue
- 6
- Pages
- 531-541
- ISSN
- 0960-7412 (ISSN-L)
- 1365-313X
- OpenAlex
- W2027317377
- Cited by
- 251
- References
- 0
- FWCI
- 5.41
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium. https://app.cochat.ai/papers/10.1046/j.1365-313x.2000.00757.x
- The paper
- Trieu, A. T., Burleigh, S. H., Kardailsky, I. V., Maldonado‐Mendoza, I. E., Versaw, W. K., Blaylock, L. A., Shin, H., Chiou, T., Katagi, H., Dewbre, G. R., Weigel, D., & Harrison, M. J. (2000). Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium. The Plant Journal. https://doi.org/10.1046/j.1365-313x.2000.00757.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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