Nicotinic receptors in the rat prefrontal cortex: increase in glutamate release and facilitation of mediodorsal thalamo‐cortical transmission
Abstract
The modulatory influence of nicotinic acetylcholine receptor (nAChRs) on thalamocortical transmission was characterized in the prelimbic area (PrL) of the rat prefrontal cortex. In the first experiment, rats received a unilateral excitotoxic lesion centred on the mediodorsal thalamic nucleus (MD), and were sacrificed 1 week later. The lesion resulted in a 40% reduction of 3H-nicotine autoradiographic labelling in the ipsilateral prefrontal cortex, particularly in areas that are innervated by the MD. Electrophysiological experiments were subsequently performed in non-lesioned anaesthetized animals, in order to study modulation of short- and long-latency responses of PrL neurons evoked by electrical stimulation of the MD. The short-latency responses result from activation of the MD-PrL pathway and are mediated via AMPA-type glutamatergic receptors, whereas the long-latency responses reflect activation of the recurrent collaterals of cortical pyramidal neurons, Iontophoretic application of nicotinic agonists (nicotine, DMPP) facilitated both types of response. Local application of the nAChR antagonists dihydro-beta-erythroidine, mecamylamine and methyllycaconitine, prevented both kinds of facilitation. Finally, intracerebral microdialysis experiments were performed in order to test for nicotinic modulation of extracellular glutamate concentrations in the PrL. Direct application of nicotine via the dialysis probe increased glutamate levels in a dose-dependent manner. This effect…
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In layer V pyramidal neurons, activation of presynaptic β2* nAChRs on thalamic glutamatergic inputs strongly enhances neuronal activity.
“In contrast, in layer V pyramidal neurons, activation of presynaptic β2* nAChRs on glutamatergic inputs from the thalamus strongly enhances activity of these neurons (Gioanni et al. 1999; Lambe et al. 2003; Couey et al. 2007; Poorthuis et al. 2012).”
From Short- and Long-Term Consequences of Nicotine Exposure during Adolescence for Prefrontal Cortex Neuronal Network Function (2012) · cites this paper as Gioanni et al. 1999
In the prefrontal cortex, nAChR expression is found across all layers.
“In the PFC, nAChR expression is found across all layers (Gioanni et al. 1999; Poorthuis et al. 2012).”
From Short- and Long-Term Consequences of Nicotine Exposure during Adolescence for Prefrontal Cortex Neuronal Network Function (2012) · cites this paper as Gioanni et al. 1999
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References 59 · Cited by 191
- Authors
- Gioanni, Y., Rougeot, C., Clarke, P. B. S., Lepousé, C., Thierry, A. M., & Vidal, C.
- Affiliations
- Inserm (FR)
- Institut Pasteur (FR)
- Corresponding author
- Gioanni, Y.
- ORCID
- Clarke, P. B. S.
- Venue
- European Journal of Neuroscience
- Publisher
- Wiley
- Published
- 1999-01
- Volume
- 11
- Issue
- 1
- Pages
- 18-30
- ISSN
- 0953-816X (ISSN-L)
- 1460-9568
- OpenAlex
- W2121254518
- Cited by
- 191
- References
- 59
- Citations by year
- 2025: 2 · 2023: 6 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 4 · 2017: 4 · 2016: 2 · 2015: 5 · 2014: 14 · 2013: 7 · 2012: 20
- FWCI
- 1.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Institut National de la Santé et de la Recherche Médicale
- Medical Research Council Canada
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Keywords
- nicotinic acetylcholine receptors, prefrontal cortex, mediodorsal thalamus, thalamocortical transmission, glutamate release, AMPA receptors, excitotoxic lesions, nicotinic agonists, nicotinic antagonists, dihydro-beta-erythroidine, mecamylamine, methyllycaconitine, nicotine
- MeSH terms
- Aconitine, Animals, Autoradiography, Cerebral Cortex, Dihydro-beta-Erythroidine, Dimethylphenylpiperazinium Iodide, Insecticides, Locomotion, Male, Mecamylamine, Neural Pathways, Synaptic Transmission, Neurons, Afferent, Nicotine, Reaction Time, Receptors, Nicotinic, Thalamus, Tritium, Rats, Sprague-Dawley, Prefrontal Cortex, Microdialysis, Excitatory Amino Acid Antagonists, Glutamic Acid, Nicotinic Agonists, Nicotinic Antagonists, 6-Cyano-7-nitroquinoxaline-2,3-dione, Excitatory Postsynaptic Potentials, Rats, Long-Evans, Rats
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