The τ Protein in Human Cerebrospinal Fluid in Alzheimer's Disease Consists of Proteolytically Derived Fragments
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Abstract: Previous studies have shown that the levels of the microtubule‐associated protein τ in the CSF of patients with Alzheimer's disease (AD) are elevated compared with age‐matched controls. In spite of these findings, the nature of τ in CSF has not been well documented. In the present study, τ was immunoprecipitated from CSF of patients with AD or acute stroke, as well as normal elderly controls, followed by immunoblot analysis. In all cases, CSF τ consisted primarily of a band migrating at 26–28 kDa. In AD and stroke patients, several smaller τ fragments were also detected. No intact τ was detected in any of the CSF samples examined. Further immunoprecipitation studies showed that the majority of the τ fragments contained the amino terminus of the molecule. Treatment of CSF τ with alkaline phosphatase did not alter the electrophoretic properties of the fragments. These studies clearly demonstrate that CSF τ is truncated rather than intact.
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Both full-length and truncated tau isoforms can be secreted.
“Both full-length and truncated tau isoforms can be secreted.”
From Molecular and cellular mechanisms underlying the pathogenesis of Alzheimer’s disease (2020) · cites this paper as [216]
In CSF, full-length tau can only be detected by western blot, but not by other methods such as ELISA and IP-MS.
“In CSF, full-length tau can only be detected by western blot, but not by other methods such as ELISA and IP-MS [216–219].”
From Molecular and cellular mechanisms underlying the pathogenesis of Alzheimer’s disease (2020) · cites this paper as [216]
Only the existence of truncated tau in CSF has been confirmed.
“However, only the existence of truncated tau in CSF has been confirmed [216–218]”
From Molecular and cellular mechanisms underlying the pathogenesis of Alzheimer’s disease (2020) · cites this paper as [216]
The presence of full-length tau in the CSF is questionable.
“whereas the presence of full-length tau in the CSF is questionable.”
From Molecular and cellular mechanisms underlying the pathogenesis of Alzheimer’s disease (2020) · cites this paper as [216]
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Cited by 162
Research works printed but not linked: 1
- McKhann G., Drachman D.. Folstein M., Katzman R., Price 0., and j. Neurochem., Vol. 68, No. I, /997 14714159, 1997, 1, Downloaded from https://onlinelibrary.wiley.com/doi/10.1046/j.1471-4159.1997.68010430.x by Mount Vernon Nazarene University, Wiley Online Library on [17/08/2026]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License · DOI
- Authors
- Johnson, G. V. W., Seubert, P., Cox, T. M., Motter, R., Brown, J. P., & Galasko, D.
- Affiliations
- University of Alabama at Birmingham (US)
- University of California San Diego (US)
- Corresponding author
- Galasko, Douglas
- Venue
- Journal of Neurochemistry
- Publisher
- Wiley
- Published
- 1997-01
- Volume
- 68
- Issue
- 1
- Pages
- 430-433
- ISSN
- 0022-3042 (ISSN-L)
- 1471-4159
- OpenAlex
- W2075560127
- Cited by
- 162
- References
- 0
- Citations by year
- 2026: 1 · 2024: 5 · 2023: 1 · 2022: 4 · 2021: 11 · 2020: 7 · 2019: 10 · 2018: 10 · 2017: 10 · 2016: 4 · 2015: 7 · 2014: 5 · 2013: 2 · 2012: 6
- FWCI
- 5.29
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Neurochemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute on Aging (AG05131, AG 12396, P50AG005131, R01AG012396)
- National Institute of Neurological Disorders and Stroke (NS-27538)
- Topics
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- tau protein, cerebrospinal fluid, Alzheimer's disease, N-terminal truncation, acute stroke, microtubule-associated proteins, alkaline phosphatase
- MeSH terms
- Alzheimer Disease, Cerebrovascular Disorders, Enzyme-Linked Immunosorbent Assay, Humans, Peptide Fragments, Peptide Hydrolases, Precipitin Tests, Reference Values, Immunoblotting, tau Proteins
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