The DOI matches its Crossref record; 53 of the 54 references it cites were found in a registry or an index.
Proinflammatory cytokines promote glial heme oxygenase-1 expression and mitochondrial iron deposition: implications for multiple sclerosis
Abstract
Proinflammatory cytokines, pathological iron deposition, and oxidative stress have been implicated in the pathogenesis of multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). HO-1 mRNA levels and mitochondrial uptake of [(55)Fe]Cl(3)-derived iron were measured in rat astroglial cultures exposed to interleukin-1beta (IL-1beta) or tumor necrosis factor-alpha (TNF-alpha) alone or in combination with the heme oxygenase-1 (HO-1) inhibitors, tin mesoporphyrin (SnMP) or dexamthasone (DEX), or interferon beta1b (INF-beta). HO-1 expression in astrocytes was evaluated by immunohistochemical staining of spinal cord tissue derived from MS and control subjects. IL-1beta or TNF-alpha promoted sequestration of non-transferrin-derived (55)Fe by astroglial mitochondria. HO-1 inhibitors, mitochondrial permeability transition pore (MTP) blockers and antioxidants significantly attenuated cytokine-related mitochondrial iron sequestration in these cells. IFN-beta decreased HO-1 expression and mitochondrial iron sequestration in IL-1beta- and TNF-alpha-challenged astroglia. The percentage of astrocytes coexpressing HO-1 in affected spinal cord from MS patients (57.3% +/- 12.8%) was significantly greater (p < 0.05) than in normal spinal cord derived from controls subjects (15.4% +/- 8.4%). HO-1 is over-expressed in MS spinal cord astroglia and may promote mitochondrial iron deposition in MS plaques. In MS, IFN-beta may attenuate glial HO-1 gene induction and aberrant mitochondrial iron deposition accruing from exposure to proinflammatory cytokines.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Incubation with interferon β reverted the cytokine-dependent induction of HO-1 and the subsequent incorporation of iron into mitochondria.162”
From The role of iron in brain ageing and neurodegenerative disorders (2014) · cites this paper as [162]
In the citing paper
“Increased HO-1 expression promoted the sequestration of non-transferrin iron into the astroglial mitochondrial compartment, as assayed by 55Fe incorporation.”
From The role of iron in brain ageing and neurodegenerative disorders (2014) · cites this paper as [162]
In the citing paper
“In-vitro studies of rat astroglial cultures showed that proinflammatory cytokines (ie, interleukin 1β and tumour necrosis factor α) increased HO-1 expression after 6 days of incubation.”
From The role of iron in brain ageing and neurodegenerative disorders (2014) · cites this paper as [162]
Citations
Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,515
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Feinberg, A. P., & Vogelstein, B. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90418-9 · Cited by 24,512
Church, G. M., & Gilbert, W. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.7.1991 · Cited by 8,213
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
(1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90381-6 · Cited by 4,952
Receptor-mediated endocytosis of transferrin in K562 cells.
Klausner, R. D., van Renswoude, J., Ashwell, G., Kempf, C., Schechter, A. N., Dean, A., & Bridges, K. R. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32481-5 · Cited by 563
(1994) · Toxicology and Industrial Health · doi:10.1177/074823379401000603 · Cited by 501
Bernardi, P. (1996) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/0005-2728(96)00041-2 · Cited by 412
The small heat-shock protein αB-crystallin as candidate autoantigen in multiple sclerosis
van Noort, J. M., van Sechel, A. C., Bajramovic, J. J., Ouagmiri, M. E., Polman, C. H., Lassmann, H., & Ravid, R. (1995) · Nature · doi:10.1038/375798a0 · Cited by 402
Expression of heme oxygenase‐1 in the senescent and alzheimer‐diseased brain
Schipper, H. M., Cissé, S., & Stopa, E. G. (1995) · Annals of Neurology · doi:10.1002/ana.410370609 · Cited by 353
Self-citationInduction of Haem Oxygenase as a Defence Against Oxidative Stress
Stocker, R. (1990) · Free Radical Research Communications · doi:10.3109/10715769009148577 · Cited by 323
Research works printed but not linked: 3
- 10.1016/0003-2697(87)90021-2 · DOI
- Paty D. W., 1998, Multiple Sclerosis, 427
- Trapp B. D., 1995, Cellular mechanisms of demyelination in multiple sclerosis brains, J. Neurochem., 64
Cited by
Sorted by citations
The role of iron in brain ageing and neurodegenerative disorders
Ward, R. J., Zucca, F. A., Duyn, J. H., Crichton, R. R., & Zecca, L. (2014) · The Lancet Neurology · doi:10.1016/s1474-4422(14)70117-6 · Cited by 1,819
The physiology and pathophysiology of nitric oxide in the brain
Guix, F. X., Uribesalgo, I., Coma, M., & Muñoz, F. J. (2005) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2005.06.001 · Cited by 723
Pathology of Demyelinating Diseases
Popescu, B. F. G., & Lucchinetti, C. F. (2011) · Annual Review of Pathology Mechanisms of Disease · doi:10.1146/annurev-pathol-011811-132443 · Cited by 426
Radical changes in multiple sclerosis pathogenesis
van Horssen, J., Witte, M. E., Schreibelt, G., & de Vries, H. E. (2010) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2010.06.011 · Cited by 325
van Horssen, J., Schreibelt, G., Drexhage, J., Hazes, T., Dijkstra, C. D., van der Valk, P., & de Vries, H. E. (2008) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2008.09.023 · Cited by 321
Metadata
- Authors
- Mehindate, K., Sahlas, D. J., Frankel, D., Mawal, Y., Liberman, A., Corcos, J., Dion, S., & Schipper, H. M.
- Affiliations
- Jewish General Hospital (CA)
- McGill University (CA)
- Corresponding author
- Schipper, H. M.
- Venue
- Journal of Neurochemistry
- Publisher
- Wiley
- Published
- 2001-06
- Volume
- 77
- Issue
- 5
- Pages
- 1386-1395
- ISSN
- 0022-3042 (ISSN-L)
- 1471-4159
- OpenAlex
- W1902487112
- Cited by
- 129
- References
- 54
- Citations by year
- 2025: 1 · 2024: 4 · 2023: 5 · 2022: 1 · 2021: 1 · 2020: 4 · 2019: 2 · 2018: 3 · 2017: 7 · 2016: 2 · 2015: 5 · 2014: 7 · 2013: 3 · 2012: 3
- FWCI
- 2.79
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neurochemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fonds de Recherche du Québec - Santé
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heme Oxygenase-1 and Carbon Monoxide
- Health Sciences › Medicine › Clinical Neurology › Multiple Sclerosis Research Studies
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Keywords
- heme oxygenase-1, multiple sclerosis, inflammatory cytokines, IL-1β, TNF-α, astrocytes, iron sequestration, interferon-beta, experimental autoimmune encephalomyelitis, oxidative stress, mitochondrial permeability transition pore, tin mesoporphyrin, dexamethasone
- MeSH terms
- Animals, Cells, Cultured, Chlorides, Encephalomyelitis, Autoimmune, Experimental, Female, Ferric Compounds, Heme Oxygenase (Decyclizing), Humans, Immunohistochemistry, Interleukin-1, Iron, Iron Radioisotopes, Mitochondria, Multiple Sclerosis, Neuroglia, RNA, Messenger, Spinal Cord, Subcellular Fractions, Transferrin, Tumor Necrosis Factor-alpha, Blotting, Northern, Cytokines, Rats, Sprague-Dawley, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
