The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Enhanced Vascularization of Cultured Skin Substitutes Genetically Modified to Overexpress Vascular Endothelial Growth Factor11The authors declared in writing to have no conflict of interest.
Open accessAbstract
Cultured skin substitutes have been used as adjunctive therapies in the treatment of burns and chronic wounds, but they are limited by lack of a vascular plexus. This deficiency leads to greater time for vascularization compared with native skin autografts and contributes to graft failure. Genetic modification of cultured skin substitutes to enhance vascularization could hypothetically lead to improved wound healing. To address this hypothesis, human keratinocytes were genetically modified by transduction with a replication incompetent retrovirus to overexpress vascular endothelial growth factor, a specific and potent mitogen for endothelial cells. Cultured skin substitutes consisting of collagen-glycosaminoglycan substrates inoculated with human fibroblasts and either vascular endothelial growth factor-modified or control keratinocytes were prepared, and were cultured in vitro for 21 d. Northern blot analysis demonstrated enhanced expression of vascular endothelial growth factor mRNA in genetically modified keratinocytes and in cultured skin substitutes prepared with modified cells. Furthermore, the vascular endothelial growth factor-modified cultured skin substitutes secreted greatly elevated levels of vascular endothelial growth factor protein throughout the entire culture period. The bioactivity of vascular endothelial growth factor protein secreted by the genetically modified cultured skin substitutes was demonstrated using a microvascular endothelial cell growth assay.…
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“While many attempts to generate complex 3D skin equivalents have achieved vascularization, hair follicle generation, and immune cell co‐cultures, there has been a complete absence of endogenously differentiating a vascularized, hair‐bearing human skin equivalent that contains immune cell populations.[ 21 , 22 , 23 , 24 , 25 , 26 , 27 ]”
From Coordinated Development of Immune Cell Populations in Vascularized Skin Organoids from Human Induced Pluripotent Stem Cells (2025) · cites this paper as [22]
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Engineered skin substitutes: practices and potentials
Supp, D. M., & Boyce, S. T. (2005) · Clinics in Dermatology · doi:10.1016/j.clindermatol.2004.07.023 · Cited by 519
Self-citationGrowth factors in the treatment of diabetic foot ulcers
Bennett, S. P., Griffiths, G. D., Schor, A. M., Leese, G., & Schor, S. L. (2003) · British journal of surgery · doi:10.1002/bjs.4019 · Cited by 335
The Pivotal Role of Vascularization in Tissue Engineering
Auger, F. A., Gibot, L., & Lacroix, D. A. (2013) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-071812-152428 · Cited by 352
Advances in tissue engineering
Langer, R., & Vacanti, J. P. (2015) · Journal of Pediatric Surgery · doi:10.1016/j.jpedsurg.2015.10.022 · Cited by 327
Tissue engineering of cultured skin substitutes
Horch, R. E., Kopp, J., Kneser, U., Beier, J. P., & Bach, A. D. (2005) · Journal of Cellular and Molecular Medicine · doi:10.1111/j.1582-4934.2005.tb00491.x · Cited by 298
Metadata
- Authors
- Supp, D. M., Supp, A. P., Boyce, S. T., & Bell, S. M.
- Affiliations
- Shriners Hospitals for Children - Cincinnati (US)
- Cincinnati Children's Hospital Medical Center (US)
- Corresponding author
- Boyce, Steven T.
- Venue
- Journal of Investigative Dermatology
- Publisher
- Elsevier BV
- Published
- 2000-01
- Volume
- 114
- Issue
- 1
- Pages
- 5-13
- ISSN
- 0022-202X (ISSN-L)
- OpenAlex
- W2010067741
- Cited by
- 120
- References
- 38
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 2 · 2023: 1 · 2021: 2 · 2020: 1 · 2019: 4 · 2018: 1 · 2017: 2 · 2016: 3 · 2015: 3 · 2014: 2 · 2013: 7 · 2012: 5
- FWCI
- 10.45
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Investigative Dermatology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Wound Healing and Treatments
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Keywords
- cultured skin substitutes, vascular endothelial growth factor, retroviral transduction, graft vascularization, wound healing, burns, chronic wounds, skin blood vessels, full-thickness skin graft, athymic mice
- MeSH terms
- Animals, Blood Vessels, Cells, Cultured, Collagen, Fibroblasts, Genetic Techniques, Glycosaminoglycans, Humans, Lymphokines, Mice, Nude, RNA, Messenger, Skin, Wounds and Injuries, Keratinocytes, Endothelial Growth Factors, Skin, Artificial, Vascular Endothelial Growth Factors, Vascular Endothelial Growth Factor A, Mice
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