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Cited by 174Mast cells and histamineSystemic Sclerosis and Related DiseasesWound Healing and Treatments

The DOI matches its Crossref record; 68 of the 70 references it cites were found in a registry or an index.

The Differentiation and Function of Myofibroblasts is Regulated by Mast Cell Mediators

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Gailit, James, Marchese, Mary J., Kew, Richard R., Gruber, Barry L.·2001·Journal of Investigative Dermatology
DOI 10.1046/j.1523-1747.2001.15211.x
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Abstract

Myofibroblasts are fibroblasts that express certain features of smooth muscle differentiation. Increased numbers of myofibroblasts and mast cells are frequently found together in a wide variety of settings, such as normal wound repair and scleroderma skin, which suggests that mediators produced by the mast cells could play a role in the regulation of myofibroblast differentiation and function. We used a human mast cell line, HMC-1, to determine if mast cells can induce normal human dermal fibroblasts to differentiate into functional myofibroblasts in vitro. We monitored the differentiation process by assaying two properties of the myofibroblast phenotype: expression of alpha-smooth muscle actin and functional capacity to contract a collagen matrix. In both a simple coculture system and in a skin-equivalent culture system, HMC-1 cells induced alpha-smooth muscle actin expression by fibroblasts. HMC-1 cells also stimulated fibroblast contraction of collagen gels, and the relative amount of contraction was dependent upon the number of HMC-1 cells present. To characterize the individual contributions made by specific mast cell products, we examined the effects of histamine, tumor necrosis factor alpha, and tryptase. Histamine induced a clear increase in alpha-smooth muscle actin expression, but it did not appear to stimulate fibroblast contraction. Tumor necrosis factor alpha had no effect in either assay. Purified human tryptase induced alpha-smooth muscle actin expression, and blocking the proteolytic activity of tryptase with specific inhibitors reduced that response. Tryptase inhibitors also eliminated the ability of HMC-1 cells to stimulate fibroblast contraction, suggesting that tryptase secreted by the HMC-1 cells may be one of the active mast cell mediators.

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  1. Ronnov-Jessen, 1993, Induction of α-smooth muscle actin by transforming growth factor-β1 in quiescent human breast gland fibroblasts, Lab Invest, 68, 696

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Metadata

Authors
Gailit, J., Marchese, M. J., Kew, R. R., & Gruber, B. L.
Affiliations
  • Northport VA Medical Center (US)
  • State University of New York (US)
Corresponding author
Gruber, Barry L.
ORCID
Venue
Journal of Investigative Dermatology
Publisher
Elsevier BV
Published
2001-11
Volume
117
Issue
5
Pages
1113-1119
ISSN
0022-202X (ISSN-L)
DOI
10.1046/j.1523-1747.2001.15211.x
OpenAlex
W2082391434
Cited by
174
References
70
Citations by year
2026: 2 · 2025: 4 · 2024: 8 · 2023: 3 · 2022: 5 · 2021: 4 · 2020: 9 · 2019: 5 · 2018: 11 · 2017: 2 · 2016: 7 · 2015: 6 · 2014: 11 · 2013: 20 · 2012: 8
FWCI
1.62
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Investigative Dermatology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
  • Health Sciences › Medicine › Rheumatology › Systemic Sclerosis and Related Diseases
  • Health Sciences › Medicine › Surgery › Wound Healing and Treatments
Keywords
myofibroblasts, mast cells, mast cell mediators, fibroblast differentiation, alpha-smooth muscle actin, collagen gel contraction, tryptase, histamine, TNF-α, HMC-1 cells, dermal fibroblasts, systemic sclerosis, wound healing
MeSH terms
Actins, Cell Differentiation, Cell Line, Collagen, Fibroblasts, Histamine, Humans, Mast Cells, Muscle, Smooth, Organ Culture Techniques, Serine Endopeptidases, Tumor Necrosis Factor-alpha, Tryptases

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