Expression of Peroxisome Proliferator-Activated Receptor and CCAAT/Enhancer Binding Protein Transcription Factors in Cultured Human Sebocytes
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Lipid synthesis and accumulation represent a major step in sebocyte differentiation and it may be of importance for sebocytes to express two families of transcription factors, CCAAT/enhancer binding proteins (c/EBPs) and peroxisome proliferator-activated receptors (PPARs), which were found to play a crucial role in the differentiation of adipocytes. Using the immortalized human sebaceous gland cell line SZ95 we examined the expression of the molecules before and after treatment with testosterone, 5alpha-dihydrotestosterone, dexamethasone, 17beta-estradiol and genistein, at 6, 12, 24, and 48 h, respectively. Reverse transcription-PCR analysis showed expression of peroxisome proliferator-activated receptors -alpha, -delta, -gamma1, -gamma2 and CCAAT/enhancer binding proteins-alpha, -beta, -gamma-delta in native SZ95 sebocytes. In western blot studies, high levels of CCAAT/enhancer binding proteins-alpha and -beta, and peroxisome proliferator-activated receptors-gamma were expressed at 6, 24, and 12 h, respectively. Immunostaining of the cultured sebocytes showed the CCAAT/enhancer binding proteins-alpha and -beta mainly localized within nuclei, whereas peroxisome proliferator-activated receptors-gamma in the cytoplasm. Strong staining of sebocytes was immunohistochemically revealed in the basal layer of sebaceous glands in human scalp and sebaceous nevus. Genistein down-regulated the expression of CCAAT/enhancer binding proteins-alpha and -beta, and peroxisome proliferator-activated receptors-gamma on the protein level. Treatment with linoleic acid for 48 h induced further differentiation of sebocytes leading to abundant lipid synthesis.
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PPARs are prime members with varying effects on sebocyte proliferation and lipogenesis.
“are the prime members71 with varying effects on sebocyte proliferation and lipogenesis.72”
From Sebaceous-immunobiology is orchestrated by sebum lipids (2017) · cites this paper as [71]
The Discussion (page not specified) reports differing effects on lipid production: linoleic acid, a PPAR-δ ligand, stimulated it, while the tested PPAR-α and PPAR-γ ligands did not. The paper does not establish varying effects of PPARs on sebocyte proliferation, so that part of the claim is unsupported. (LLM, conf=0.86)
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References 26 · Cited by 157
- Authors
- Chen, W., Yang, C.-C., Sheu, H.-M., Seltmann, H., & Zouboulis, C. C.
- Affiliations
- National Cheng Kung University (TW)
- Freie Universität Berlin (DE)
- Corresponding author
- Chen, WenChieh
- Venue
- Journal of Investigative Dermatology
- Publisher
- Elsevier BV
- Published
- 2003-09
- Volume
- 121
- Issue
- 3
- Pages
- 441-447
- ISSN
- 0022-202X (ISSN-L)
- OpenAlex
- W2093351574
- Cited by
- 157
- References
- 26
- Citations by year
- 2025: 3 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 3 · 2020: 4 · 2019: 3 · 2018: 3 · 2017: 7 · 2016: 4 · 2015: 11 · 2014: 12 · 2013: 4 · 2012: 10
- FWCI
- 2.88
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Investigative Dermatology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Chicago
- National Science Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Health Sciences › Medicine › Dermatology › Acne and Rosacea Treatments and Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- sebocytes, peroxisome proliferator-activated receptors, C/EBP, sebocyte differentiation, sebaceous glands, testosterone, dihydrotestosterone, dexamethasone, estradiol, genistein, linoleic acid, lipogenesis, sebaceous nevus, transcription factor expression
- MeSH terms
- Antineoplastic Agents, Cell Differentiation, Cell Line, Transformed, Dexamethasone, Estrogens, Glucocorticoids, Humans, RNA, Messenger, Sebaceous Glands, Gonadal Steroid Hormones, Dihydrotestosterone, Testosterone, Transcription Factors, Gene Expression, Receptors, Cytoplasmic and Nuclear, Genistein, CCAAT-Enhancer-Binding Proteins, CCAAT-Enhancer-Binding Protein-alpha, CCAAT-Enhancer-Binding Protein-beta, CCAAT-Enhancer-Binding Protein-delta
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