Iron and 8-isoprostane levels in acute and chronic wounds
Open accessAbstract
The purpose of this study was to determine differences in iron and iron protein (ferritin and transferrin) levels in chronic venous ulcers and acute wounds. The deleterious effect of iron in free-radical-induced tissue damage was indirectly examined by assessing 8-isoprostane levels and antioxidant status in wound fluid samples. Wound fluid samples from chronic leg ulcers in nonhealing and healing phases and wound fluid from mastectomy wounds were assayed for ferritin, transferrin, total iron, 8-isoprostane, and total antioxidant status. Immunohistochemistry and Perls' staining were performed on paired biopsies from chronic leg ulcers and on normal skin biopsies. Chronic wound fluid had significantly greater levels of ferritin (p < 0.05) and lower levels of transferrin (p < 0.001) than acute wound fluid and there was a significant reduction in the level of ferritin in healing compared to nonhealing chronic leg ulcers (p < 0.05). No significant differences were observed in the levels of total iron present in the wound fluids. Histologic staining showed consistently more ferritin and ferric iron in chronic wound tissue than in normal skin. Elevated levels of 8-isoprostane and antioxidants were observed for chronic wound fluid compared to acute wound fluid (p < 0.001). These results suggest the existence of an environment of oxidative stress in chronic wounds and the likely contribution of iron to exacerbating tissue damage and delaying healing in these wounds.
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Wenk et al. and Yeoh-Ellerton found increased iron levels in exudates from chronic leg ulcers as compared to acute wounds, and observed significant concentrations of metabolites from oxidative stress.
“Wenk et al.7 and Yeoh-Ellerton13 found increased iron levels in exudates from chronic leg ulcers as compared to acute wounds. They also observed significant concentrations of metabolites from oxidative stress.7,13”
From The Big Idea: Iron-Dependent Inflammation in Venous Disease and Proposed Parallels in Multiple Sclerosis (2006) · cites this paper as [13]
Intra- and extra-cellular overload of iron in the tissue could potentially be dangerous for generation of free radicals due to possible release of free iron from deposits.
“Intra- and extra-cellular overload of iron in the tissue could potentially be dangerous for generation of free radicals due to possible release of free iron from deposits.4-9,13,14”
From The Big Idea: Iron-Dependent Inflammation in Venous Disease and Proposed Parallels in Multiple Sclerosis (2006) · cites this paper as [13]
Our findings support the hypothesis that lesions are promoted by enhanced iron release and reactive oxygen species generation.
“Our findings support the hypothesis that lesions are promoted by enhanced iron release and ROS generation.4-9,13,14,16”
From The Big Idea: Iron-Dependent Inflammation in Venous Disease and Proposed Parallels in Multiple Sclerosis (2006) · cites this paper as [13]
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References 61 · Cited by 125
- Authors
- Yeoh-Ellerton, S., & Stacey, M. C.
- Affiliations
- The University of Western Australia (AU)
- Corresponding author
- Stacey, Michael C.
- ORCID
- Stacey, Michael C.
- Venue
- Journal of Investigative Dermatology
- Publisher
- Elsevier BV
- Published
- 2003-10
- Volume
- 121
- Issue
- 4
- Pages
- 918-925
- ISSN
- 0022-202X (ISSN-L)
- OpenAlex
- W2157072884
- Cited by
- 125
- References
- 61
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 7 · 2023: 4 · 2022: 6 · 2021: 4 · 2020: 4 · 2019: 6 · 2018: 4 · 2017: 6 · 2016: 7 · 2015: 4 · 2014: 10 · 2013: 6 · 2012: 4
- FWCI
- 4.16
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Investigative Dermatology (publishedVersion)
- PubMed (publishedVersion)
- UWA Profiles and Research Repository (UWA) (submittedVersion)
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
- Keywords
- chronic wounds, chronic venous leg ulcers, acute wounds, wound fluid, ferritin, transferrin, iron, 8-isoprostane, oxidative stress, antioxidant status, wound healing, free radicals, tissue damage
- MeSH terms
- Acute Disease, Adult, Aged, Aged, 80 and over, Antioxidants, Biopsy, Chronic Disease, Female, Ferritins, Ferrozine, Humans, Indicators and Reagents, Iron, Male, Middle Aged, Transferrin, Varicose Ulcer, Lipid Peroxidation, Dinoprost, Oxidative Stress, F2-Isoprostanes, Extracellular Fluid
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