The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Age Related Changes in 5‐methylcytosine Content in Human Peripheral Leukocytes and Placentas: an HPLC‐based Study
Abstract
Summary: The goal of the present study was to investigate inter‐individual and age‐dependent variation of global DNA methylation in human tissues. In this work, we examined 5‐methyldeoxycytidine (met C) content by HPLC in human peripheral blood leukocytes obtained from 76 healthy individuals of ages varying from 4 to 94 years (yr), and 39 human placentas from various gestational stages. The HPLC analysis revealed a significant variation of met C across individuals and is consistent with the previous findings of age‐dependent decrease of global methylation levels in human tissues. The age‐dependent decrease of met C was relatively small, but statistically highly significant (p= 0.0002) in the aged group (65.9 ± 8.9 [mean age ± SD] yr; n = 22) in comparison to the young adult group (19.3 ± 1.4 yr; n = 21). Males showed a subtle but statistically significant higher mean met C content than females. In contrast to the peripheral blood samples, DNA extracted from placentas exhibited gestational stage‐dependent increase of methylation levels that appeared to inversely correlate with the expression levels of human endogenous retroviruses. These data may be helpful in further studies of DNA methylation, such as inheritance of epigenetic patterns, environment‐induced changes, and involvement of epigenetic changes in disease.
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Cites
DNA methylation patterns and epigenetic memory
Bird, A. (2002) · Genes & Development · doi:10.1101/gad.947102 · Cited by 7,124
Epigenetic inheritance at the agouti locus in the mouse
Morgan, H. D., Sutherland, H. G. E., Martin, D. I. K., & Whitelaw, E. (1999) · Nature Genetics · doi:10.1038/15490 · Cited by 1,476
Transcription of IAP endogenous retroviruses is constrained by cytosine methylation
Walsh, C. P., Chaillet, J. R., & Bestor, T. H. (1998) · Nature Genetics · doi:10.1038/2413 · Cited by 1,105
Metastable epialleles in mammals
Rakyan, V. K., Blewitt, M. E., Druker, R., Preis, J. I., & Whitelaw, E. (2002) · Trends in Genetics · doi:10.1016/s0168-9525(02)02709-9 · Cited by 499
Suzuki, T., Fujii, M., & Ayusawa, D. (2002) · Experimental Gerontology · doi:10.1016/s0531-5565(02)00061-x · Cited by 62
Cited by
Rapid DNA methylation changes after exposure to traffic particles
Baccarelli, A., Wright, R. O., Bollati, V., Tarantini, L., Litonjua, A. A., Suh, H. H., Zanobetti, A., Sparrow, D., Vokonas, P. S., & Schwartz, J. (2009) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.200807-1097oc · Cited by 653
Aging and epigenetic drift: a vicious cycle
Issa, J.-P. (2014) · Journal of Clinical Investigation · doi:10.1172/jci69735 · Cited by 419
Endogenous retroviral LTRs as promoters for human genes: A critical assessment
Cohen, C. J., Lock, W. M., & Mager, D. L. (2009) · Gene · doi:10.1016/j.gene.2009.06.020 · Cited by 314
The role of epigenetics in aging and age-related diseases
Calvanese, V., Lara, E., Kahn, A., & Fraga, M. F. (2009) · Ageing Research Reviews · doi:10.1016/j.arr.2009.03.004 · Cited by 329
Making sense of the ageing methylome
Seale, K., Horvath, S., Teschendorff, A., Eynon, N., & Voisin, S. (2022) · Nature Reviews Genetics · doi:10.1038/s41576-022-00477-6 · Cited by 274
Metadata
- Authors
- Fuke, C., Shimabukuro, M., Petronis, A., Sugimoto, J., Oda, T., Miura, K., Miyazaki, T., Ogura, C., Okazaki, Y., & Jinno, Y.
- Venue
- Annals of Human Genetics
- Publisher
- Wiley
- Published
- 2004-05
- Volume
- 68
- Issue
- 3
- Pages
- 196-204
- ISSN
- 0003-4800 (ISSN-L)
- 1469-1809
- OpenAlex
- W2028418054
- Cited by
- 401
- References
- 5
- FWCI
- 2.84
- Type
- journal article
- Language
- en
- Open access
- closed
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- Fuke, C., Shimabukuro, M., Petronis, A., Sugimoto, J., Oda, T., Miura, K., Miyazaki, T., Ogura, C., Okazaki, Y., & Jinno, Y. (2004). Age Related Changes in 5‐methylcytosine Content in Human Peripheral Leukocytes and Placentas: an HPLC‐based Study. Annals of Human Genetics. https://doi.org/10.1046/j.1529-8817.2004.00081.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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