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Cited by 113Cell Adhesion Molecules ResearchLiver physiology and pathologyCellular Mechanics and Interactions

Expression and function of integrin receptors for collagen and laminin in cultured human hepatic stellate cells

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Carloni, V, Romanelli, RG, Pinzani, M, Laffi, G, Gentilini, P·1996·Gastroenterology
DOI 10.1053/gast.1996.v110.pm8613002
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Abstract

Background & aims Human hepatic stellate cells (HSCs), liver-specific pericytes, are currently considered major producers of extracellular matrix (ECM) components and key elements in the development of liver fibrogenesis. However, little is known about the possible functional interactions between HSCs and the various ECM components. Therefore, the present study was designed to evaluate the expression of integrins, the major family of extracellular matrix receptors. Methods Integrin expression was evaluated by immunoprecipitation and confirmed by immunocytochemistry and flow cytometry. Results Human HSCs were shown to express alpha1beta1, alpha2beta1, alpha(v)beta1. Adhesion to type IV collagen, type I collagen, fibronectin, and laminin 1 was inhibited by anti-beta1 antibody identifying beta1-containing integrins as possible receptors for these components. In addition, we showed that HSCs express alpha6beta4, a heterodimer known to mediate adhesion of epithelial cells to laminin and not previously characterized in mesenchymal cells. Adhesion to laminin 1 was not inhibited by antibodies specific for alpha6 or beta4, thus establishing that laminin 1 is not a ligand for alpha6beta4 in this cell type. Conclusions These findings represent the first description of integrin receptors in HSC and provide an attempt to cover the gap of information in the field of HSC-ECM interactions.

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Authors
Carloni, V, Romanelli, R., Pinzani, M., Laffi, G., & Gentilini, P.
Affiliations
University of Florence (IT)
ORCID
Venue
Gastroenterology
Publisher
Elsevier BV
Published
1996-04
Volume
110
Issue
4
Pages
1127-1136
ISSN
0016-5085 (ISSN-L)
DOI
10.1053/gast.1996.v110.pm8613002
OpenAlex
W2111868375
Cited by
113
References
0
Citations by year
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FWCI
3.31
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Gastroenterology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
hepatic stellate cells, integrins, collagen, laminin, alpha1beta1 integrin, alpha2beta1 integrin, alpha5beta1 integrin, alpha6beta4 integrin, extracellular matrix receptors, cell adhesion, liver fibrosis, extracellular matrix
MeSH terms
Cell Adhesion, Cells, Cultured, Collagen, Extracellular Matrix, Fibronectins, Humans, Immunohistochemistry, Laminin, Liver, Precipitin Tests, Integrins, Receptors, Laminin, Adipocytes, Receptors, Collagen

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