The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Injection of Cultured Cells with a ROCK Inhibitor for Bullous Keratopathy
Abstract
BACKGROUND: Corneal endothelial cell (CEC) disorders, such as Fuchs's endothelial corneal dystrophy, induce abnormal corneal hydration and result in corneal haziness and vision loss known as bullous keratopathy. We investigated whether injection of cultured human CECs supplemented with a rho-associated protein kinase (ROCK) inhibitor into the anterior chamber could increase CEC density. METHODS: passaged cells were supplemented with a ROCK inhibitor (final volume, 300 μl) and injected into the anterior chamber of the eye that was selected for treatment. After the procedure, patients were placed in a prone position for 3 hours. The primary outcome was restoration of corneal transparency, with a CEC density of more than 500 cells per square millimeter at the central cornea at 24 weeks after cell injection. Secondary outcomes were a corneal thickness of less than 630 μm and an improvement in best corrected visual acuity equivalent to two lines or more on a Landolt C eye chart at 24 weeks after cell injection. RESULTS: At 24 weeks after cell injection, we recorded a CEC density of more than 500 cells per square millimeter (range, 947 to 2833) in 11 of the 11 treated eyes (100%; 95% confidence interval [CI], 72 to 100), of which 10 had a CEC density exceeding 1000 cells per square millimeter. A corneal thickness of less than 630 μm (range, 489 to 640) was attained in 10 of the 11 treated eyes (91%; 95% CI, 59 to 100), and an improvement in best corrected visual acuity of two lines or more was recorded in 9 of the 11 treated eyes (82%; 95% CI, 48 to 98). CONCLUSIONS: Injection of human CECs supplemented with a ROCK inhibitor was followed by an increase in CEC density after 24 weeks in 11 persons with bullous keratopathy. (Funded by the Japan Agency for Medical Research and Development and others; UMIN number, UMIN000012534 .).
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Global Survey of Corneal Transplantation and Eye Banking
Gain, P., Jullienne, R., He, Z., Aldossary, M., Acquart, S., Cognasse, F., & Thuret, G. (2016) · JAMA Ophthalmology · doi:10.1001/jamaophthalmol.2015.4776 · Cited by 1,674
Ocular immune privilege: therapeutic opportunities from an experiment of nature
Streilein, J. W. (2003) · Nature Reviews Immunology · doi:10.1038/nri1224 · Cited by 807
Tan, D. T., Dart, J. K., Holland, E. J., & Kinoshita, S. (2012) · The Lancet · doi:10.1016/s0140-6736(12)60437-1 · Cited by 799
Self-citationHolladay, J. T. (2004) · Journal of Cataract and Refractive Surgery · doi:10.1016/j.jcrs.2004.01.014 · Cited by 686
Descemet's Membrane Endothelial Keratoplasty
Price, M. O., Giebel, A. W., Fairchild, K. M., & Price, F. W. (2009) · Ophthalmology · doi:10.1016/j.ophtha.2009.07.010 · Cited by 654
Mesenchymal to Epithelial Transition in Development and Disease
Chaffer, C. L., Thompson, E. W., & Williams, E. D. (2007) · Cells Tissues Organs · doi:10.1159/000101298 · Cited by 324
Amniotic Membrane as a Carrier for Cultivated Human Corneal Endothelial Cell Transplantation
Ishino, Y., Sano, Y., Nakamura, T., Connon, C. J., Rigby, H., Fullwood, N. J., & Kinoshita, S. (2004) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.03-0016 · Cited by 316
Self-citationEnhancement on Primate Corneal Endothelial Cell Survival In Vitro by a ROCK Inhibitor
Okumura, N., Ueno, M., Koizumi, N., Sakamoto, Y., Hirata, K., Hamuro, J., & Kinoshita, S. (2009) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.08-2634 · Cited by 300
Self-citationPreliminary Clinical Results of Descemet Membrane Endothelial Keratoplasty
Melles, G. R. J., Ong, T. S., Ververs, B., & van der Wees, J. (2008) · American Journal of Ophthalmology · doi:10.1016/j.ajo.2007.09.021 · Cited by 286
Cultured Human Corneal Endothelial Cell Transplantation with a Collagen Sheet in a Rabbit Model
Mimura, T., Yamagami, S., Yokoo, S., Usui, T., Tanaka, K., Hattori, S., Irie, S., Miyata, K., Araie, M., & Amano, S. (2004) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.03-1174 · Cited by 277
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Hos, D., Matthaei, M., Bock, F., Maruyama, K., Notara, M., Clahsen, T., Hou, Y., Le, V. N. H., Salabarria, A.-C., Horstmann, J., et al. (2019) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2019.07.001 · Cited by 303
Fuchs endothelial corneal dystrophy: The vicious cycle of Fuchs pathogenesis
Ong Tone, S., Kocaba, V., Böhm, M., Wylegala, A., White, T. L., & Jurkunas, U. V. (2021) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2020.100863 · Cited by 284
Scioli, M. G., Storti, G., D’Amico, F., Rodríguez Guzmán, R., Centofanti, F., Doldo, E., Céspedes Miranda, E. M., & Orlandi, A. (2020) · Journal of Clinical Medicine · doi:10.3390/jcm9061995 · Cited by 281
Rho-kinase ROCK inhibitors reduce oligomeric tau protein
Hamano, T., Shirafuji, N., Yen, S.-H., Yoshida, H., Kanaan, N. M., Hayashi, K., Ikawa, M., Yamamura, O., Fujita, Y., Kuriyama, M., et al. (2020) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2019.12.009 · Cited by 76
Effect of a p38 Mitogen-Activated Protein Kinase Inhibitor on Corneal Endothelial Cell Proliferation
Nakahara, M., Okumura, N., Nakano, S., & Koizumi, N. (2018) · Investigative Opthalmology & Visual Science · doi:10.1167/iovs.18-24394 · Cited by 30
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Metadata
- Authors
- Kinoshita, S., Koizumi, N., Ueno, M., Okumura, N., Imai, K., Tanaka, H., Yamamoto, Y., Nakamura, T., Inatomi, T., Bush, J., Toda, M., Hagiya, M., Yokota, I., Teramukai, S., Sotozono, C., & Hamuro, J.
- Venue
- New England Journal of Medicine
- Publisher
- Massachusetts Medical Society
- Published
- 2018-03-15
- Volume
- 378
- Issue
- 11
- Pages
- 995-1003
- ISSN
- 0028-4793 (ISSN-L)
- 1533-4406
- OpenAlex
- W2793941328
- Cited by
- 542
- References
- 27
- FWCI
- 33.70
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kinoshita, S., Koizumi, N., Ueno, M., Okumura, N., Imai, K., Tanaka, H., Yamamoto, Y., Nakamura, T., Inatomi, T., Bush, J., Toda, M., Hagiya, M., Yokota, I., Teramukai, S., Sotozono, C., & Hamuro, J. (2018). Injection of Cultured Cells with a ROCK Inhibitor for Bullous Keratopathy. New England Journal of Medicine. https://doi.org/10.1056/nejmoa1712770
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