The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Characterization of Exocellular Protein and Its Role in Bioflocculation
Abstract
The relationship between exocellular biopolymer concentration and cation concentration was examined using laboratory scale activated sludge reactors with bactopeptone as a feed. An increase in the divalent cation concentration in the feed to the reactors was associated with an increase in the bound exocellular protein concentration, and high sodium concentrations resulted in a decrease in the bound protein concentration. The changes in bound biopolymer were explained according to the cation bridging model. Incubation of a laboratory activated sludge with a proteolytic enzyme resulted in deflocculation of the suspension as measured by an increase in the number of particles in the 5–40 μm range, which suggested that the exocellular protein was strongly involved in the aggregation of bacteria into flocs. SDS PAGE results revealed the presence of a single protein in the exocellular biopolymer extract from municipal, industrial, and laboratory activated sludge samples. The molecular weight of the protein was approximately 15 daltons. Amino acid analysis and amino acid sequencing results suggested the protein was a lectinlike protein, and binding site inhibition studies demonstrated that the protein had lectinlike activity.
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Flemming, H.-C., & Wingender, J. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2415 · Cited by 10,137
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A review on anaerobic membrane bioreactors: Applications, membrane fouling and future perspectives
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Metadata
- Authors
- Higgins, M. J., & Novak, J. T.
- Venue
- Journal of Environmental Engineering
- Published
- 1997-05
- Volume
- 123
- Issue
- 5
- Pages
- 479-485
- ISSN
- 0733-9372 (ISSN-L)
- 1943-7870
- DOI
- 10.1061/(asce)0733-9372(1997)123:5(479
- OpenAlex
- W2104153603
- Cited by
- 408
- References
- 14
- FWCI
- 6.14
- Type
- journal article
- Language
- en
- Open access
- closed
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- Higgins, M. J., & Novak, J. T. (1997). Characterization of Exocellular Protein and Its Role in Bioflocculation. Journal of Environmental Engineering. https://doi.org/10.1061/(asce)0733-9372(1997)123%3A5(479
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