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Real-time investigation of dynamic protein crystallization in living cells

Schönherr, R., Klinge, M., Rudolph, J. M., Fita, K., Rehders, D., Lübber, F., Schneegans, S., Majoul, I. V., Duszenko, M., Betzel, C., Brandariz-Nuñez, A., Martinez-Costas, J., Duden, R., Redecke, L.·2015·Structural Dynamics
DOI 10.1063/1.4921591PMC PMC4711630
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Abstract

X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro.

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Metadata

Authors
Schönherr, R., Klinge, M., Rudolph, J. M., Fita, K., Rehders, D., Lübber, F., Schneegans, S., Majoul, I. V., Duszenko, M., Betzel, C., Brandariz-Nuñez, A., Martinez-Costas, J., Duden, R., & Redecke, L.
Venue
Structural Dynamics
Publisher
AIP Publishing
Published
2015-05-22
Volume
2
Issue
4
ISSN
2329-7778
DOI
10.1063/1.4921591
PMC
PMC4711630
Cited by
42
References
1
Language
en
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