Segmental allergen challenge in patients with atopic asthma leads to increased IL-9 expression in bronchoalveolar lavage fluid lymphocytes
Open accessAbstract
Background IL-9 is a T(H)2 cell-derived cytokine that might be involved in the pathophysiology of allergic diseases. Little is known about its expression and release during the allergic response in the human lung. Objective The expression of IL-9 was measured in 10 atopic subjects with mild asthma and 5 nonatopic healthy control subjects at baseline and 24 hours after segmental sham and allergen challenge. Methods IL-9 protein was measured in bronchoalveolar lavage (BAL) fluid by means of ELISA and detected within the BAL cells by means of immunocytochemistry. Furthermore, IL9 mRNA expression of BAL cells was detected by means of real-time PCR. Results Although only low or undetectable amounts of IL9 mRNA and IL-9 protein were present in nonatopic control subjects and atopic asthmatic patients at baseline, there was an increase after segmental allergen challenge in the atopic subjects. Lymphocytes were identified as major cellular sources of IL-9 production by means of immunocytochemistry. Furthermore, IL-9 protein and IL9 mRNA expression correlated with eosinophil numbers in BAL fluid. Conclusions These findings demonstrate that IL-9 is specifically upregulated after local allergen challenge in the lungs of atopic asthmatic patients. Lymphocytes are the major cellular source of IL-9. The increased expression and its correlation with eosinophil numbers suggest a potential role for IL-9 in the late phase of the allergic response.
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IL-9 expression is increased in lungs of asthmatic patients.
“IL-9 expression is increased in lungs of asthmatic patients20,21”
From The transcription factor PU.1 is required for the development of IL-9-producing T cells and allergic inflammation (2010) · cites this paper as [20]
The Discussion reports increased pulmonary IL-9 expression in atopic asthmatic patients 24 hours after segmental allergen challenge. It also says expression was low or undetectable before challenge in subjects with mild asthma, so the source does not support an unqualified increase in the lungs of asthmatic patients generally. (LLM, conf=0.89)
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- Authors
- Erpenbeck, V. J., Hohlfeld, J. M., Volkmann, B., Hagenberg, A., Geldmacher, H., Braun, A., & Krug, N.
- Affiliations
- Medizinische Hochschule Hannover (DE)
- Fraunhofer Institute for Toxicology and Experimental Medicine (DE)
- Venue
- Journal of Allergy and Clinical Immunology
- Publisher
- Elsevier BV
- Published
- 2003-06
- Volume
- 111
- Issue
- 6
- Pages
- 1319-1327
- ISSN
- 0091-6749 (ISSN-L)
- OpenAlex
- W2049848014
- Cited by
- 145
- References
- 3
- Citations by year
- 2025: 2 · 2024: 4 · 2023: 7 · 2022: 6 · 2021: 3 · 2020: 5 · 2019: 8 · 2018: 6 · 2017: 10 · 2016: 10 · 2015: 6 · 2014: 14 · 2013: 9 · 2012: 12
- FWCI
- 2.69
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Allergy and Clinical Immunology (publishedVersion)
- PubMed (publishedVersion)
- Publikationsdatenbank der Fraunhofer-Gesellschaft (Fraunhofer-Gesellschaft) (submittedVersion)
- Fraunhofer-Publica (Fraunhofer-Gesellschaft) (submittedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (SFB 587)
- Topics
- Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
- Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
- Life Sciences › Immunology and Microbiology › Immunology › IL-33, ST2, and ILC Pathways
- Keywords
- IL-9, allergic asthma, bronchoalveolar lavage fluid, eosinophils, Th2 cytokines, allergic airway inflammation, late-phase allergic response
- MeSH terms
- Adolescent, Adult, Allergens, Asthma, Bronchoalveolar Lavage Fluid, Cell Count, Eosinophilia, Female, Gene Expression Regulation, Humans, Hypersensitivity, Immediate, Immunohistochemistry, Lung, Male, RNA, Messenger, T-Lymphocytes, Up-Regulation, Polymerase Chain Reaction, Interleukin-9
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