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The hyp-1 gene is not a limiting factor for hypericin biosynthesis in the genus Hypericum
Abstract
Biosynthesis of the hypericins that accumulate in the dark glands of some members of the genus Hypericum is poorly understood. The gene named hyp-1, isolated from Hypericum perforatum L. has been proposed as playing an important role in the final steps of hypericin biosynthesis. To study the role of this candidate gene in relation to the production of hypericins, the expression of this gene was studied in 15 Hypericum species with varying ability to synthesise hypericin. While the accumulation of hypericins and emodin, an intermediate in the respective pathway, was associated with the dark glands in the hypericin-producing species, the hyp-1 gene was expressed in all studied species regardless of whether hypericins and emodin were detected in the plants. The coding sequences of hyp-1 cDNA were isolated from all species and showed more than 86% similarity to each other. Although, in general, an increased level of the hyp-1 gene transcript was detected in hypericin-producing species, several of the hypericin-lacking species expressed comparable levels as well. Our results question the role of the hyp-1 gene product as a key enzyme responsible for biosynthesis of hypericins in the genus Hypericum. The function of the hyp-1 gene may not be restricted to hypericin biosynthesis only, or some additional factors are necessary for completion of hypericin biosynthesis.
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“For example, in emodin pigment synthesis, the main precursor is the octaketide emodin anthrone, which results from a polyketide condensation reaction between acetyl-coenzyme A (-CoA) and malonyl-CoA fixed by a polyketide synthase (PKS) [74].”
From Natural Quinone Dyes: A Review on Structure, Extraction Techniques, Analysis and Application Potential (2021) · cites this paper as [74]
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“The second proposed pathway is called the shikimate or chorismate/O-succinylbenzoic acid pathway (Fig. 2b).Here, pigments eventually only have one of the rings unsubstituted and at least one hydroxyl group on the R1 (Fig. 1) position, as in alizarine, pseudopurpurin, purpurin and lucidin [74].”
From Natural Quinone Dyes: A Review on Structure, Extraction Techniques, Analysis and Application Potential (2021) · cites this paper as [74]
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Research works printed but not linked: 31
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Cited by
Dulo, B., Phan, K., Githaiga, J., Raes, K., & De Meester, S. (2021) · Waste and Biomass Valorization · doi:10.1007/s12649-021-01443-9 · Cited by 181
Metadata
- Authors
- Košuth, J., Smelcerovic, A., Borsch, T., Zuehlke, S., Karppinen, K., Spiteller, M., Hohtola, A., & Cellárová, E.
- Affiliations
- University of Pavol Jozef Šafárik (SK)
- BInstitute of Environmental Research, Technical University of Dortmund, Otto-Hahn-Str. 6, 44221 Dortmund, Germany.
- Botanic Garden and Botanical Museum Berlin (DE)
- TU Dortmund University (DE)
- University of Oulu (FI)
- AInstitute of Biology and Ecology, P. J. Šafárik University in Košice, Mánesova 23, 041 54 Košice, Slovakia.
- Venue
- Functional Plant Biology
- Publisher
- CSIRO Publishing
- Published
- 2010-12-17
- Volume
- 38
- Issue
- 1
- Pages
- 35-43
- ISSN
- 1445-4408 (ISSN-L)
- 1445-4416
- OpenAlex
- W1993997647
- Cited by
- 41
- References
- 0
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 4 · 2023: 1 · 2022: 2 · 2021: 5 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 5 · 2015: 2 · 2014: 2 · 2013: 3 · 2012: 3
- FWCI
- 0.78
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Functional Plant Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Bundesministerium für Bildung und Forschung
- Agentúra na Podporu Výskumu a Vývoja
- Vedecká Grantová Agentúra MŠVVaŠ SR a SAV
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Natural Compound Pharmacology Studies
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant tissue culture and regeneration
- Keywords
- Hypericum, Hypericum perforatum, emodin, gene expression, cDNA cloning, phylogenetic conservation, biosynthetic pathway
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- The paper
- Košuth, J., Smelcerovic, A., Borsch, T., Zuehlke, S., Karppinen, K., Spiteller, M., Hohtola, A., & Cellárová, E. (2010). The hyp-1 gene is not a limiting factor for hypericin biosynthesis in the genus Hypericum. Functional Plant Biology. https://doi.org/10.1071/fp10144
