The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
Dynamics of HIV-1 recombination in its natural target cells
Open accessAbstract
Genetic recombination is believed to assist HIV-1 diversification and escape from host immunity and antiviral therapies, yet this process remains largely unexamined within the natural target-cell populations. We developed a method for measuring HIV-1 recombination directly that employs reporter viruses bearing functional enhanced yellow fluorescent protein (YFP) and enhanced cyan fluorescent protein (CFP) genes in which recombination produces a modified GFP gene and GFP fluorescence in the infected cells. These reporter viruses allow simultaneous quantification of the dynamics of HIV-1 infection, coinfection, and recombination in cell culture and in animal models by flow-cytometric analysis. Multiround infection assays revealed that productive cellular coinfection was subject to little functional inhibition. As a result, generation of recombinants proceeded according to the square of the infection rate during HIV-1 replication in T lymphocytes and within human thymic grafts in severe combined immunodeficient (SCID)-hu (Thy/Liv) mice. These results suggest that increases in viral load may confer a compounding risk of virus escape by means of recombinational diversification. A single round of replication in T lymphocytes in culture generated an average of nine recombination events per virus, and infection of macrophages led to ≈30 crossover events, making HIV-1 up to an order of magnitude more recombinogenic than recognized previously and demonstrating that the infected cell exerts a profound influence on the frequency of recombination.
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Cited by
Domingo, E., Sheldon, J., & Perales, C. (2012) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.05023-11 · Cited by 1,009
Schaller, T., Ocwieja, K. E., Rasaiyaah, J., Price, A. J., Brady, T. L., Roth, S. L., Hué, S., Fletcher, A. J., Lee, K., KewalRamani, V. N., et al. (2011) · PLoS Pathogens · doi:10.1371/journal.ppat.1002439 · Cited by 470
The IFITM Proteins Inhibit HIV-1 Infection
Lu, J., Pan, Q., Rong, L., Liu, S.-L., & Liang, C. (2011) · Journal of Virology · doi:10.1128/jvi.01531-10 · Cited by 417
The Interferon-Inducible MxB Protein Inhibits HIV-1 Infection
Liu, Z., Pan, Q., Ding, S., Qian, J., Xu, F., Zhou, J., Cen, S., Guo, F., & Liang, C. (2013) · Cell Host & Microbe · doi:10.1016/j.chom.2013.08.015 · Cited by 334
Chan, J. M., Carlsson, G., & Rabadan, R. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1313480110 · Cited by 269
Metadata
- Authors
- Levy, D. N., Aldrovandi, G. M., Kutsch, O., & Shaw, G. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-03-09
- Volume
- 101
- Issue
- 12
- Pages
- 4204-4209
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2060534902
- Cited by
- 441
- References
- 41
- FWCI
- 9.01
- Type
- journal article
- Language
- en
- Open access
- green
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