The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A tautomeric zinc sensor for ratiometric fluorescence imaging: Application to nitric oxide-induced release of intracellular zinc
Open accessAbstract
Zinc is an essential metal ion for human growth and development, the disruption of cellular Zn 2+ homeostasis being implicated in several major disorders including Alzheimer's disease, diabetes, and cancer. The molecular mechanisms of Zn 2+ physiology and pathology are insufficiently understood, however, owing in part to the lack of tools for measuring changes in intracellular Zn 2+ concentrations with high spatial and temporal fidelity. To address this critical need, we have synthesized, characterized, and applied an intracellular fluorescent probe for the ratiometric imaging of Zn 2+ based on a tautomeric seminaphthofluorescein platform. Zin-naphthopyr 1 (ZNP1) affords single-excitation, dual-emission ratiometric detection of intracellular Zn 2+ through Zn 2+ -controlled switching between fluorescein and naphthofluorescein tautomeric forms. The probe features visible excitation and emission profiles, excellent selectivity responses for Zn 2+ over competing Ca 2+ and Mg 2+ ions at intracellular concentrations, a dissociation constant ( K d ) for Zn 2+ of <1 nM, and an 18-fold increase in fluorescence emission intensity ratio (λ 624 /λ 528 ) upon zinc binding. We demonstrate the value of the ZNP1 platform for biological applications by imaging changes in intracellular [Zn 2+ ] in living mammalian cells. Included is the ratiometric detection of endogenous pools of intracellular Zn 2+ after NO-induced release of Zn 2+ from cellular metalloproteins. We anticipate that ZNP1 and related probes should find utility for interrogating the biology of Zn 2+ .
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S-nitrosylation of matrix metalloproteinases: signaling pathway to neuronal cell death
Gu, Z., Kaul, M., Yan, B., Kridel, S. J., Cui, J., Strongin, A., Smith, J. W., Liddington, R. C., & Lipton, S. A. (2002) · Science · doi:10.1126/science.1073634 · Cited by 950
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Domaille, D. W., Que, E. L., & Chang, C. J. (2008) · Nature Chemical Biology · doi:10.1038/nchembio.69 · Cited by 1,081
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Metadata
- Authors
- Chang, C. J., Jaworski, J., Nolan, E. M., Sheng, M., & Lippard, S. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-01-20
- Volume
- 101
- Issue
- 5
- Pages
- 1129-1134
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2010935723
- Cited by
- 220
- References
- 1
- FWCI
- 7.12
- Type
- journal article
- Language
- en
- Open access
- green
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- Chang, C. J., Jaworski, J., Nolan, E. M., Sheng, M., & Lippard, S. J. (2004). A tautomeric zinc sensor for ratiometric fluorescence imaging: Application to nitric oxide-induced release of intracellular zinc. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0308079100
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