The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
A single amino acid converts a repressor to an activator of flowering
Open accessAbstract
Homologous proteins occurring through gene duplication may give rise to novel functions through mutations affecting protein sequence or expression. Comparison of such homologues allows insight into how morphological traits evolve. However, it is often unclear which changes are key to determining new functions. To address these ideas, we have studied a system where two homologues have evolved clear and opposite functions in controlling a major developmental switch. In plants, flowering is a major developmental transition that is critical to reproductive success. Arabidopsis phosphatidylethanolamine-binding protein homologues TERMINAL FLOWER 1 (TFL1) and FLOWERING LOCUS T (FT) are key controllers of flowering, determining when and where flowers are made, but as opposing functions: TFL1 is a repressor, FT is an activator. We have uncovered a striking molecular basis for how these homologous proteins have diverged. Although <60% identical, we have shown that swapping a single amino acid is sufficient to convert TFL1 to FT function and vice versa. Therefore, these key residues may have strongly contributed to the selection of these important functions over plant evolution. Further, our results suggest that TFL1 and FT are highly conserved in biochemical function and that they act as repressors or activators of flowering through discrimination of structurally related interactors by a single residue.
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Cites
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Cited by
FT Protein Acts as a Long-Range Signal in Arabidopsis
Jaeger, K. E., & Wigge, P. A. (2007) · Current Biology · doi:10.1016/j.cub.2007.05.008 · Cited by 731
Molecular mechanisms of flower development: an armchair guide
Krizek, B. A., & Fletcher, J. C. (2005) · Nature Reviews Genetics · doi:10.1038/nrg1675 · Cited by 624
Hanano, S., & Goto, K. (2011) · The Plant Cell · doi:10.1105/tpc.111.088641 · Cited by 406
Evolution of the PEBP Gene Family in Plants: Functional Diversification in Seed Plant Evolution
Karlgren, A., Gyllenstrand, N., Källman, T., Sundström, J. F., Moore, D., Lascoux, M., & Lagercrantz, U. (2011) · Plant Physiology · doi:10.1104/pp.111.176206 · Cited by 337
The FLOWERING LOCUS T-Like Gene Family in Barley (Hordeum vulgare)
Faure, S., Higgins, J., Turner, A., & Laurie, D. A. (2007) · Genetics · doi:10.1534/genetics.106.069500 · Cited by 313
Metadata
- Authors
- Hanzawa, Y., Money, T., & Bradley, D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2005-05-13
- Volume
- 102
- Issue
- 21
- Pages
- 7748-7753
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2046004083
- Cited by
- 542
- References
- 41
- FWCI
- 27.53
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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