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A label-free immunosensor array using single-chain antibody fragments
Open accessAbstract
We report a microcantilever-based immunosensor operated in static deflection mode with a performance comparable with surface plasmon resonance, using single-chain Fv (scFv) antibody fragments as receptor molecules. As a model system scFv fragments with specificity to two different antigens were applied. We introduced a cysteine residue at the C terminus of each scFv construct to allow covalent attachment to gold-coated sensor interfaces in directed orientation. Application of an array enabled simultaneous deflection measurements of sensing and reference cantilevers. The differential deflection signal revealed specific antigen binding and was proportional to the antigen concentration in solution. Using small, oriented scFv fragments as receptor molecules we increased the sensitivity of microcantilevers to ≈1 nM.
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Cancer nanotechnology: opportunities and challenges
Ferrari, M. (2005) · Nature Reviews Cancer · doi:10.1038/nrc1566 · Cited by 4,558
Systems Biology and New Technologies Enable Predictive and Preventative Medicine
Hood, L., Heath, J. R., Phelps, M. E., & Lin, B. (2004) · Science · doi:10.1126/science.1104635 · Cited by 1,108
High-affinity binders selected from designed ankyrin repeat protein libraries
Binz, H. K., Amstutz, P., Kohl, A., Stumpp, M. T., Briand, C., Forrer, P., Grütter, M. G., & Plückthun, A. (2004) · Nature Biotechnology · doi:10.1038/nbt962 · Cited by 656
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Weighing of biomolecules, single cells and single nanoparticles in fluid
Burg, T. P., Godin, M., Knudsen, S. M., Shen, W., Carlson, G., Foster, J. S., Babcock, K., & Manalis, S. R. (2007) · Nature · doi:10.1038/nature05741 · Cited by 1,213
Micro- and nanomechanical sensors for environmental, chemical, and biological detection
Waggoner, P. S., & Craighead, H. G. (2007) · Lab on a Chip · doi:10.1039/b707401h · Cited by 678
Ngoepe, M., Choonara, Y., Tyagi, C., Tomar, L., Du Toit, L., Kumar, P., Ndesendo, V., & Pillay, V. (2013) · Sensors · doi:10.3390/s130607680 · Cited by 92
Metadata
- Authors
- Backmann, N., Zahnd, C., Huber, F., Bietsch, A., Plückthun, A., Lang, H.-P., Güntherodt, H.-J., Hegner, M., & Gerber, C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2005-09-28
- Volume
- 102
- Issue
- 41
- Pages
- 14587-14592
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2126924184
- Cited by
- 284
- References
- 3
- FWCI
- 7.72
- Type
- journal article
- Language
- en
- Open access
- green
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- Backmann, N., Zahnd, C., Huber, F., Bietsch, A., Plückthun, A., Lang, H.-P., Güntherodt, H.-J., Hegner, M., & Gerber, C. (2005). A label-free immunosensor array using single-chain antibody fragments. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0504917102
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