The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa ( Medicago sativa L.)
Open accessAbstract
Improving the digestibility of forages provides a means to enhance animal performance and protect the environment against excessive animal waste. Increased lignin content during maturity, and corresponding changes in lignin composition, correlate with decreased digestibility of forages. These relationships have yet to be investigated in isogenic systems. By targeting three specific cytochrome P450 enzymes of the lignin pathway for antisense down-regulation, we generated transgenic alfalfa lines with a range of differences in lignin content and composition. There was a strong negative relationship between lignin content and rumen digestibility, but no relationship between lignin composition and digestibility, in these transgenic lines. Models for genetic manipulation of forage digestibility based on the changes in lignin composition that increase paper-pulping efficiency in trees are therefore invalid. Down-regulation of 4-coumarate 3-hydroxylase provided the largest improvements in digestibility yet seen in a forage crop.
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Cites
Colorimetric Method for Determination of Sugars and Related Substances
DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956) · Analytical Chemistry · doi:10.1021/ac60111a017 · Cited by 52,269
Cited by
Exploring bacterial lignin degradation
Brown, M. E., & Chang, M. C. (2014) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2013.11.015 · Cited by 438
Modifying crops to increase cell wall digestibility
Jung, H.-J. G., Samac, D. A., & Sarath, G. (2012) · Plant Science · doi:10.1016/j.plantsci.2011.10.014 · Cited by 167
Economical concerns of lignin in the energy sector
Sharma, A., Kaur, P., Singh, G., & Arya, S. K. (2021) · Cleaner Engineering and Technology · doi:10.1016/j.clet.2021.100258 · Cited by 38
Metadata
- Authors
- Reddy, M. S. S., Chen, F., Shadle, G., Jackson, L., Aljoe, H., & Dixon, R. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2005-11
- Volume
- 102
- Issue
- 46
- Pages
- 16573-16578
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2144423170
- Cited by
- 354
- References
- 1
- FWCI
- 3.57
- Type
- journal article
- Language
- en
- Open access
- green
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- Reddy, M. S. S., Chen, F., Shadle, G., Jackson, L., Aljoe, H., & Dixon, R. A. (2005). Targeted down-regulation of cytochrome P450 enzymes for forage quality improvement in alfalfa ( Medicago sativa L.). Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0505749102
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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