The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Spatiotemporal control of spindle midzone formation by PRC1 in human cells
Abstract
We have examined the role of PRC1, a midzone-associated, microtubule bundling, Cdk substrate protein, in regulating the spatiotemporal formation of the midzone in HeLa cells. Cdk-mediated phosphorylation of PRC1 in early mitosis holds PRC1 in an inactive monomeric state. During the metaphase-to-anaphase transition, PRC1 is dephosphorylated, promoting PRC1 oligomerization. Using time-lapse video microscopy, RNA interference, 3D immunofluorescence reconstruction imaging, and rescue experiments, we demonstrate that the dephosphorylated form of PRC1 is essential for bundling antiparallel, nonkinetochore, interdigitating microtubules to establish the midzone that is necessary for cytokinesis. Our results thus indicate that PRC1 is an essential factor in controlling the spatiotemporal formation of the midzone in human cells.
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Cites
Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation
Kurasawa, Y., Earnshaw, W. C., Mochizuki, Y., Dohmae, N., & Todokoro, K. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600347 · Cited by 333
Cited by
Partial inhibition of Cdk1 in G2phase overrides the SAC and decouples mitotic events
McCloy, R. A., Rogers, S., Caldon, C. E., Lorca, T., Castro, A., & Burgess, A. (2014) · Cell Cycle · doi:10.4161/cc.28401 · Cited by 853
Cytokinetic Abscission: Molecular Mechanisms and Temporal Control
Mierzwa, B., & Gerlich, D. W. (2014) · Developmental Cell · doi:10.1016/j.devcel.2014.11.006 · Cited by 288
Wolfe, B. A., Takaki, T., Petronczki, M., & Glotzer, M. (2009) · PLoS Biology · doi:10.1371/journal.pbio.1000110 · Cited by 225
Bastos, R. N., Cundell, M. J., & Barr, F. A. (2014) · Journal of Cell Biology · doi:10.1083/jcb.201409129 · Cited by 73
Still entangled: assembly of the central spindle by multiple microtubule modulators
Douglas, M. E., & Mishima, M. (2010) · Seminars in Cell & Developmental Biology · doi:10.1016/j.semcdb.2010.08.005 · Cited by 52
Metadata
- Authors
- Zhu, C., Lau, E., Schwarzenbacher, R., Bossy-Wetzel, E., & Jiang, W.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-04-18
- Volume
- 103
- Issue
- 16
- Pages
- 6196-6201
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2027540736
- Cited by
- 167
- References
- 1
- FWCI
- 4.49
- Type
- journal article
- Language
- en
- Open access
- closed
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- Zhu, C., Lau, E., Schwarzenbacher, R., Bossy-Wetzel, E., & Jiang, W. (2006). Spatiotemporal control of spindle midzone formation by PRC1 in human cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0506926103
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
