The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene
Abstract
Secreted parasitism proteins encoded by parasitism genes expressed in esophageal gland cells mediate infection and parasitism of plants by root-knot nematodes (RKN). Parasitism gene 16D10 encodes a conserved RKN secretory peptide that stimulates root growth and functions as a ligand for a putative plant transcription factor. We used in vitro and in vivo RNA interference approaches to silence this parasitism gene in RKN and validate that the parasitism gene has an essential function in RKN parasitism of plants. Ingestion of 16D10 dsRNA in vitro silenced the target parasitism gene in RKN and resulted in reduced nematode infectivity. In vivo expression of 16D10 dsRNA in Arabidopsis resulted in resistance effective against the four major RKN species. Because no known natural resistance gene has this wide effective range of RKN resistance, bioengineering crops expressing dsRNA that silence target RKN parasitism genes to disrupt the parasitic process represents a viable and flexible means of developing novel durable RKN-resistant crops and could provide crops with unprecedented broad resistance to RKN.
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Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
Expression profiling reveals off-target gene regulation by RNAi
Jackson, A. L., Bartz, S. R., Schelter, J., Kobayashi, S. V., Burchard, J., Mao, M., Li, B., Cavet, G., & Linsley, P. S. (2003) · Nature Biotechnology · doi:10.1038/nbt831 · Cited by 2,370
Cited by
Mao, Y.-B., Cai, W.-J., Wang, J.-W., Hong, G.-J., Tao, X.-Y., Wang, L.-J., Huang, Y.-P., & Chen, X.-Y. (2007) · Nature Biotechnology · doi:10.1038/nbt1352 · Cited by 1,358
Unravelling rootstockxscion interactions to improve food security
Albacete, A., Martinez-Andujar, C., Martinez-Perez, A., Thompson, A. J., Dodd, I. C., & Perez-Alfocea, F. (2015) · Journal of Experimental Botany · doi:10.1093/jxb/erv027 · Cited by 299
Metadata
- Authors
- Huang, G., Allen, R., Davis, E. L., Baum, T. J., & Hussey, R. S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-09-26
- Volume
- 103
- Issue
- 39
- Pages
- 14302-14306
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2122443974
- Cited by
- 625
- References
- 2
- FWCI
- 34.08
- Type
- journal article
- Language
- en
- Open access
- closed
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- Huang, G., Allen, R., Davis, E. L., Baum, T. J., & Hussey, R. S. (2006). Engineering broad root-knot resistance in transgenic plants by RNAi silencing of a conserved and essential root-knot nematode parasitism gene. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0604698103
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