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Engineering RNA sequence specificity of Pumilio repeats
Open accessAbstract
Puf proteins bind RNA sequence specifically and regulate translation and stability of target mRNAs. A “code” for RNA recognition has been deduced from crystal structures of the Puf protein, human Pumilio1, where each of eight repeats binds an RNA base via a combination of three side chains at conserved positions. Here, we report the creation of seven soluble mutant proteins with predictably altered sequence specificity, including one that binds tightly to adenosine-uracil-rich element RNA. These data show that Pumilio1 can be used as a scaffold to engineer RNA-binding proteins with designed sequence specificity.
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Cites
Alternative splicing in disease and therapy
Garcia-Blanco, M. A., Baraniak, A. P., & Lasda, E. L. (2004) · Nature Biotechnology · doi:10.1038/nbt964 · Cited by 527
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Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors
Konermann, S., Lotfy, P., Brideau, N. J., Oki, J., Shokhirev, M. N., & Hsu, P. D. (2018) · Cell · doi:10.1016/j.cell.2018.02.033 · Cited by 1,164
Imaging dynamics of endogenous mitochondrial RNA in single living cells
Ozawa, T., Natori, Y., Sato, M., & Umezawa, Y. (2007) · Nature Methods · doi:10.1038/nmeth1030 · Cited by 282
Human Pumilio Proteins Recruit Multiple Deadenylases to Efficiently Repress Messenger RNAs
Van Etten, J., Schagat, T. L., Hrit, J., Weidmann, C. A., Brumbaugh, J., Coon, J. J., & Goldstrohm, A. C. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.373522 · Cited by 205
Advances in CRISPR-Cas systems for RNA targeting, tracking and editing
Wang, F., Wang, L., Zou, X., Duan, S., Li, Z., Deng, Z., Luo, J., Lee, S. Y., & Chen, S. (2019) · Biotechnology Advances · doi:10.1016/j.biotechadv.2019.03.016 · Cited by 128
Metadata
- Authors
- Cheong, C.-G., & Hall, T. M. T.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2006-09-12
- Volume
- 103
- Issue
- 37
- Pages
- 13635-13639
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1999269806
- Cited by
- 230
- References
- 1
- FWCI
- 3.32
- Type
- journal article
- Language
- en
- Open access
- green
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