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Structural basis for PRYSPRY-mediated tripartite motif (TRIM) protein function
Open accessAbstract
The human tripartite motif (TRIM) family comprises 70 members, including HIV restriction factor TRIM5α and disease-associated proteins TRIM20 (pyrin) and TRIM21. TRIM proteins have conserved domain architecture but diverse cellular roles. Here, we describe how the C-terminal PRYSPRY domain mediates diverse TRIM functions. The crystal structure of TRIM21 PRYSPRY in complex with its target IgG Fc reveals a canonical binding interface comprised of two discrete pockets formed by antibody-like variable loops. Alanine scanning of this interface has identified the hot-spot residues that control TRIM21 binding to Fc; the same hot-spots control HIV/murine leukemia virus restriction by TRIM5α and mediate severe familial Mediterranean fever in TRIM20/pyrin. Characterization of the IgG binding site for TRIM21 PRYSPRY reveals TRIM21 as a superantigen analogous to bacterial protein A and suggests that an antibody bipolar bridging mechanism may contribute to the pathogenic accumulation of anti-TRIM21 autoantibody immune complex in autoimmune disease.
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“The SPRY domain of TRIM21, a major autoantigen in autoimmune diseases, was solved in complex with the Fc region of the immunoglobulin IgG 126 , revealing a well-defined pocket at the IgG binding site”
From Targeted protein degradation: expanding the toolbox (2019) · cites this paper as [126]
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Ozato, K., Shin, D., Chang, T.-H., & Morse, H. C. (2008) · Nature reviews. Immunology · doi:10.1038/nri2413 · Cited by 1,131
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Metadata
- Authors
- James, L. C., Keeble, A. H., Khan, Z., Rhodes, D. A., & Trowsdale, J.
- Affiliations
- MRC Laboratory of Molecular Biology (GB)
- University of Cambridge (GB)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2007-04-10
- Volume
- 104
- Issue
- 15
- Pages
- 6200-6205
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC1851072
- OpenAlex
- W2022754521
- Cited by
- 386
- References
- 50
- Citations by year
- 2026: 20 · 2025: 26 · 2024: 27 · 2023: 25 · 2022: 22 · 2021: 24 · 2020: 26 · 2019: 27 · 2018: 11 · 2017: 18 · 2016: 17 · 2015: 15 · 2014: 19 · 2013: 19 · 2012: 18
- FWCI
- 13.29
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Medical Research Council
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Monoclonal and Polyclonal Antibodies Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Inflammasome and immune disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
- Keywords
- tripartite motif proteins, PRYSPRY domain, TRIM21, TRIM5α, IgG Fc binding, protein crystal structure, familial Mediterranean fever, superantigens, autoantibodies, immune complexes, autoimmune disease, protein-protein interaction
- MeSH terms
- SS-A Antigen, Amino Acid Sequence, Autoantibodies, Autoimmune Diseases, Binding Sites, Antibody, Calorimetry, Cell Line, Crystallography, DNA-Binding Proteins, Fluorescence Polarization, Multigene Family, Humans, Immunoglobulin G, Models, Molecular, Molecular Sequence Data, Nuclear Proteins, Protein Binding, Protein Conformation, Ribonucleoproteins, Structure-Activity Relationship, Gene Expression, Sequence Alignment, Protein Structure, Tertiary
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