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Arabidopsis fhl / fhy1 double mutant reveals a distinct cytoplasmic action of phytochrome A
Open accessAbstract
Phytochrome A (phyA) plays an important role during germination and early seedling development. Because phyA is the primary photoreceptor for the high-irradiance response and the very-low-fluence response, it can trigger development not only in red and far-red (FR) light but also in a wider range of light qualities. Although phyA action is generally associated with translocation to the nucleus and regulation of transcription, there is evidence for additional cytoplasmic functions. Because nuclear accumulation of phyA has been shown to depend on far-red-elongated hypocotyl 1 (FHY1) and FHL (FHY1-like), investigation of phyA function in a double fhl/fhy1 mutant might be valuable in revealing the mechanism of phyA translocation and possible cytoplasmic functions. In fhl/fhy1 , the FR-triggered nuclear translocation of phyA could no longer be detected but could be restored by transgenic expression of CFP:FHY1 . Whereas the fhl/fhy1 mutant showed a phyA phenotype in respect to hypocotyl elongation and cotyledon opening under high-irradiance response conditions as well as a typical phyA germination phenotype under very-low-fluence response conditions, fhl/fhy1 showed no phenotype with respect to the phyA-dependent abrogation of negative gravitropism in blue light and in red-enhanced phototropism, demonstrating clear cytoplasmic functions of phyA. Disturbance of phyA nuclear import in fhl/fhy1 led to formation of FR-induced phyA:GFP cytoplasmic foci resembling the sequestered areas of phytochrome. FHY1 and FHL play crucial roles in phyA nuclear translocation and signaling. Thus the double-mutant fhl/fhy1 allows nuclear and cytoplasmic phyA functions to be separated, leading to the novel identification of cytoplasmic phyA responses.
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“Red light-activated phytochromes can inhibit blue light-induced migration of PHOT1 to the cytoplasm. (Rosler et al., 2007; Han et al., 2008).”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Rosler et al., 2007
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Cites
HY4 gene of A. thaliana encodes a protein with characteristics of a blue-light photoreceptor
Ahmad, M., & Cashmore, A. R. (1993) · Nature · doi:10.1038/366162a0 · Cited by 1,279
Reed, J. W., Nagpal, P., Poole, D. S., Furuya, M., & Chory, J. (1993) · The Plant Cell · doi:10.1105/tpc.5.2.147 · Cited by 957
Signal Transduction in Maize and Arabidopsis Mesophyll Protoplasts
Sheen, J. (2001) · PLANT PHYSIOLOGY · doi:10.1104/pp.010820 · Cited by 693
Clack, T., Mathews, S., & Sharrock, R. (1994) · Plant Molecular Biology · doi:10.1007/bf00043870 · Cited by 678
Deng, X., Caspar, T., & Quail, P. H. (1991) · Genes & Development · doi:10.1101/gad.5.7.1172 · Cited by 564
Shinomura, T., Nagatani, A., Hanzawa, H., Kubota, M., Watanabe, M., & Furuya, M. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.15.8129 · Cited by 549
Phytochrome A null mutants of Arabidopsis display a wild-type phenotype in white light.
Whitelam, G. C., Johnson, E., Peng, J., Carol, P., Anderson, M. L., Cowl, J. S., & Harberd, N. P. (1993) · The Plant Cell · doi:10.1105/tpc.5.7.757 · Cited by 535
Lin, C., Yang, H., Guo, H., Mockler, T. C., Chen, J., & Cashmore, A. R. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.5.2686 · Cited by 519
Functional selectivity of recombinant mammalian SWI/SNF subunitsPreprint
Kadam, S., McAlpine, G. S., Phelan, M. L., Kingston, R. E., Jones, K. A., & Emerson, B. M. (2000) · Genes & Development · doi:10.1101/gad.828000 · Cited by 496
Multiple transcription-factor genes are early targets of phytochrome A signaling
Tepperman, J. M., Zhu, T., Chang, H., Wang, X., & Quail, P. H. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.161300998 · Cited by 481
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Research works printed but not linked: 6
- S Kircher, L Kozma-Bognar, L Kim, E Adam, K Harter, E Schafer, F Nagy Plant Cell 11, 1445–1456 (1999).
- GC Whitelam, E Johnson, J Peng, P Carol, ML Anderson, JS Cowl, NP Harberd Plant Cell 5, 757–768 (1993).
- CD Fairchild, MA Schumaker, PH Quail Genes Dev 14, 2377–2391 (2000).
- PH Quail Encyclopedia of Plant Physiol, eds W Shropshire, H Mohr (Springer, Heidelberg) Vol 16, 178–212, Book A. (1983).
- JW Reed, P Nagpal, DS Poole, M Furuya, J Chory Plant Cell 5, 147–157 (1993).
- , eds J Sambrook, D Russel (Cold Spring Harbor Lab Press, Plainview, NY) Molecular Cloning: A Laboratory Manual, pp. A9.28 (2001).
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Analysis of phytochrome signaling pathways in Zea mays
Kumar, I. (2012) · Illinois Digital Environment for Access to Learning and Scholarship (University of Illinois at Urbana-Champaign)
Phototropin 1 and dim-blue light modulate the red light de-etiolation response
Wang, Y., & Folta, K. M. (2014) · Plant Signaling & Behavior · doi:10.4161/15592324.2014.976158
Missense Mutation in the Amino Terminus of Phytochrome A Disrupts the Nuclear Import
Sokolova, V., & Nagy, F. (2012)
Mechanisms of the phototropic pathway
Morrow, J. (2018) · doi:10.32469/10355/69866
Xiang, Z., Qingping, Z., Xu, Y., Mu, S.-C., Xiao, Z., 赵, 翔., 赵, 青., 杨, 煦., 慕, 世., & 张, 骁. (2015) · Chinese Bulletin of Botany · doi:10.3724/sp.j.1259.2015.00122
Gallemí, M. (2013) · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas)
A Fast and Reliable Nuclei Extraction Method for Phytochrome Functional Analysis
Villmann, I., Nagatani, A., & Zeidler, M. (2025) · Methods in molecular biology · doi:10.1007/978-1-0716-4791-2_20
Self-citationUnraveling the Role of DcPHYA in Carotenoid Synthesis in Carrot TaprootsPreprint
Quian-Ulloa, R., Arancibia, N., González-Calquín, C., Munizaga, F., Medina, E., & Stange, C. (2025) · SSRN Electronic Journal · doi:10.2139/ssrn.5767226
Liu, P. (2011) · Montana State University ScholarWorks (Montana State University)
Yin, L., Zeng, H., Jia, X., Zhao, Z., Wang, Z., Musazade, E., Liu, Y., Xu, M. Y., Lu, J., Guo, L., et al. (2025) · Plants · doi:10.3390/plants15010028
Jarosz-Ostrówka, M., Kwaśniak-Owczarek, M., Małecka, N., Mielczarek, M., Migdał, P., Gagliardi, O., Lijsebettens, M. V., Mackiewicz, P., & Wołoszyńska, M. (2026) · Plant and Cell Physiology · doi:10.1093/pcp/pcag005
Quian-Ulloa, R., Arancibia, N., González-Calquín, C., Munizaga, F., Vargas, M., Medina, E., & Stange, C. (2026) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2026.111247
Feng, X., Wang, H., Huo, C., Liu, Y., Zhang, Y., Lv, X., Guo, Y., & Zhu, P. (2026) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2026.111494
Metadata
- Authors
- Rösler, J., Klein, I., & Zeidler, M.
- Affiliations
- Justus-Liebig-Universität Gießen (DE)
- Carnegie Institution for Science (US)
- Corresponding author
- Zeidler, Mathias
- ORCID
- Zeidler, Mathias
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2007-06-19
- Volume
- 104
- Issue
- 25
- Pages
- 10737-10742
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC1965582
- OpenAlex
- W2096295687
- Cited by
- 114
- References
- 47
- Citations by year
- 2026: 3 · 2025: 7 · 2023: 4 · 2022: 5 · 2021: 5 · 2020: 3 · 2019: 4 · 2018: 7 · 2017: 1 · 2016: 4 · 2015: 3 · 2014: 10 · 2013: 8 · 2012: 13
- FWCI
- 10.94
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Keywords
- phytochrome A, nuclear translocation, far-red light, high irradiance response, hypocotyl elongation, cotyledon opening, gravitropism, phototropism, Arabidopsis thaliana, double mutants
- MeSH terms
- Cytoplasm, Mutation, Recombinant Fusion Proteins, Arabidopsis, Hypocotyl, Transgenes, Arabidopsis Proteins, Plants, Genetically Modified, Green Fluorescent Proteins, Phytochrome A
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